Gas7 Is a Novel Dendritic Spine Initiation Factor

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2023-04
Major/Subject
Mcode
Degree programme
Language
en
Pages
21
1-21
Series
eNeuro, Volume 10, issue 4
Abstract
Brain stores new information by modifying connections between neurons. When new information is learnt, a group of neurons gets activated and they are connected to each other via synapses. Dendritic spines are pro-trusions along neuronal dendrites where excitatory synapses are located. Dendritic spines are the first struc-tures to protrude out from the dendrite to reach out to other neurons and establish a new connection. Thus, it is expected that neuronal activity enhances spine initiation. However, the molecular mechanisms linking neuronal activity to spine initiation are poorly known. Membrane binding BAR domain proteins are involved in spine initiation, but it is not known whether neuronal activity affects BAR domain proteins. Here, we used bicuculline treatment to activate excitatory neurons in organotypic hippocampal slices. With this experimental setup, we identified F-BAR domain containing growth arrest-specific protein (Gas7) as a novel spine initiation factor responding to neuron activity. Upon bicuculline addition, Gas7 clustered to create spine initiation hotspots, thus increasing the probability to form new spines in activated neurons. Gas7 clustering and localization was dependent on PI3-kinase (PI3K) activity and intact F-BAR domain. Gas7 overexpression enhanced N-WASP localization to clusters as well as it increased the clustering of actin. Arp2/3 complex was required for normal Gas7-induced actin clustering. Gas7 overexpression increased and knock-down decreased spine density in hippocampal pyramidal neurons. Taken together, we suggest that Gas7 creates platforms under the dendritic plasma membrane which facilitate spine initiation. These platforms grow on neuronal activation, increasing the probability of making new spines and new connections between active neurons. As such, we identified a novel molecular mechanism to link neuronal activity to the formation of new connections between neurons.
Description
Funding Information: This work was funded by Academy of Finland Grant SA 307735 (to P.H.); the Doctoral Programme Brain & Mind, University of Helsinki (P.K.); the United Kingdom Dementia Research Institute partner funders (Medical Research Council, Alzheimer’s Research United Kingdom, and the Alzheimer’s Society; P.O.); the Dementia Australia Research Foundation (DARF) Back Block Bards Project Grant (P.O.); and the National Health and Medical Research Council (NHMRC) Project Grant (P.O.). Publisher Copyright: © 2023 Khanal et al.
Keywords
actin cytoskeleton, BAR domains, dendritic spines, spine initiation
Other note
Citation
Khanal, P, Boskovic, Z, Lahti, L, Ghimire, A, Minkeviciene, R, Opazo, P & Hotulainen, P 2023, ' Gas7 Is a Novel Dendritic Spine Initiation Factor ', eNeuro, vol. 10, no. 4, ENEURO.0344-22.2023, pp. 1-21 . https://doi.org/10.1523/ENEURO.0344-22.2023