The Identification of Ethidium Bromide-Degrading Bacteria from Laboratory Gel Electrophoresis Waste

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2022-02-24
Major/Subject
Mcode
Degree programme
Language
en
Pages
9
Series
BioTech, Volume 11, issue 1
Abstract
Ethidium bromide (EtBr) is widely used in most laboratories to detect nucleic acids in gel electrophoresis applications. It is a well-known carcinogenic and mutagenic agent, which can affect biotic components of the place in which it is disposed. Usually the gel-waste is either buried in the ground or incinerated, whereas the liquid waste is disposed of down the sink following the recommended methods of treatment. The recommended methods do not involve biological potential, but rather make use of chemicals, which may further deteriorate soil and water quality. The present study identifies and characterizes the EtBr-degrading bacterial isolates BR3 and BR4. A bibliographic review of the risk status of using these isolates for the treatment of lab waste in laboratory settings is also presented. BR3 was identified as Proteus terrae N5/687 (LN680103) and BR4 as Morganella morganii subsp. morganii ATCC 25830 (AJ301681) with 99.9% and 99.48% similarity, respec-tively, using an EzBioCloud microbial identifier. The literature revealed the bacterium Proteus terrae as a non-pathogenic and natural microflora of humans, but Morganella morganii as an opportunistic pathogen. These organisms belong to risk group II. Screening the sensitivity of these isolates to antibiotics revealed a sufficient number of antibiotics, which can be used to control them, if required. BR3 and BR4 exhibited resistance to individual antibiotics, ampicillin and vancomycin, whereas only BR3 was resistant to tetracycline. The current investigation, along with earlier reported work on these isolates, identifies BR3 as a useful isolate in the industrial application for the degradation of EtBr. Identical and related microorganisms, which are available in the culture collection repositories, can also be explored for such potential to formulate a microbial consortium for the bioremediation of ethidium bromide prior to its disposal.
Description
Funding Information: The authors V.P.G. and A.K. are thankful to DBT [BT/PR9028/INF/22/193/2013] for their support to establish BUILDER program at Central University of Jharkhand and UGC for providing a platform to support research. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords
16S-rRNA, bioaccumulation, biotransformation, inhibition zone, Morganella morganii, pathogenicity, Proteus terrae, risk assessment
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Citation
Gandhi , V P , Kesari , K K & Kumar , A 2022 , ' The Identification of Ethidium Bromide-Degrading Bacteria from Laboratory Gel Electrophoresis Waste ' , BioTech , vol. 11 , no. 1 , 4 . https://doi.org/10.3390/BIOTECH11010004