Fast HILIC method for separation and quantification of non-volatile aromatic compounds and monosaccharides from willow (Salix sp.) bark extract
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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8
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Applied Sciences, Volume 11, issue 9
Abstract
Willow bark water extracts contain a mixture of chemically heterogeneous compounds. Fast screening techniques of the extracts are often needed to obtain information on the profile of bioactive and/or other valuable components in the extract. This is, however, a challenging task due to the different chemical structures of the components. Willow bark extract from the hybrid Karin contains several bioactive compounds such as aromatic picein, triandrin, and (+)-catechin. Willow bark extract also contains significant amounts of the monosaccharides fructose and glucose. Here, we demonstrate the applicability of hydrophilic interaction liquid chromatography, coupled with evaporative light scattering and ultraviolet detectors, for the simultaneous separation and quantification of major aromatic compounds and monosaccharides from the willow bark extract. The ternary eluent mixture consisting of acetonitrile, water, and methanol enabled the baseline separation of the main components in the extract in a short analysis time, which makes this method ideal for fast screening of the plant extracts and investigating the purity of fractionated bioactive compounds.Description
Funding Information: The authors thank Yuki Tobimatsu and John Ralph from University of Wisconsin-Madison for the triandrin sample. We thank Jari Heinonen from LUT University for information regarding the limits of quantification for monosaccharides using the chromatographic system with RI detection. The authors thank Rita Hatakka from Aalto University and Jarl Hemming from ?bo Akademi for assistance with the HPAEC-PAD and GC-FID techniques. This work was part of the Academy of Finland?s Flagship Programme under Projects No. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). This work made use of Aalto University?s Bioeconomy Facilities. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
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Meriö-Talvio, H, Dou, J, Vuorinen, T & Pitkänen, L 2021, 'Fast HILIC method for separation and quantification of non-volatile aromatic compounds and monosaccharides from willow ( Salix sp.) bark extract', Applied Sciences, vol. 11, no. 9, 3808. https://doi.org/10.3390/app11093808