Disposable Nafion-Coated Single-Walled Carbon Nanotube Test Strip for Electrochemical Quantitative Determination of Acetaminophen in a Finger-Prick Whole Blood Sample

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2020-10-06
Major/Subject
Mcode
Degree programme
Language
en
Pages
8
13017-13024
Series
Analytical Chemistry, Volume 92, issue 19
Abstract
A disposable electrochemical test strip for the quantitative point-of-care (POC) determination of acetaminophen (paracetamol) in plasma and finger-prick whole blood was fabricated. The industrially scalable dry transfer process of single-walled carbon nanotubes (SWCNTs) and screen printing of silver were combined to produce integrated electrochemical test strips. Nafion coating stabilized the potential of the Ag reference electrode and enabled the selective detection in spiked plasma as well as in whole blood samples. The test strips were able to detect acetaminophen in small 40 mu L samples with a detection limit of 0.8 mu M and a wide linear range from 1 mu M to 2 mM, well within the required clinical range. After a simple 1:1 dilution of plasma and whole blood, a quantitative detection with good recoveries of 79% in plasma and 74% in whole blood was achieved. These results strongly indicate that these electrodes can be used directly to determine the unbound acetaminophen fraction without the need for any additional steps. The developed test strip shows promise as a rapid and simple POC quantitative acetaminophen assay.
Description
Keywords
SIMULTANEOUS VOLTAMMETRIC DETERMINATION, DOPED DIAMOND ELECTRODE, PARACETAMOL, FILM, SENSOR, ACID, HEPATOTOXICITY, NANOCOMPOSITE, NANOPARTICLES, FABRICATION
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Citation
Wester, N, Mikladal, B F, Varjos, I, Peltonen, A, Kalso, E, Lilius, T, Laurila, T & Koskinen, J 2020, ' Disposable Nafion-Coated Single-Walled Carbon Nanotube Test Strip for Electrochemical Quantitative Determination of Acetaminophen in a Finger-Prick Whole Blood Sample ', Analytical Chemistry, vol. 92, no. 19, pp. 13017-13024 . https://doi.org/10.1021/acs.analchem.0c01857