Effect of Glycosylation on Self-Assembly of Lipid A Lipopolysaccharides in Aqueous Solutions
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2023-06-20
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en
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7
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Langmuir, Volume 39, issue 24, pp. 8516-8522
Abstract
Lipopolysaccharides (LPSs) based on lipid A produced by bacteria are of interest due to their bioactivity in stimulating immune responses, as are simpler synthetic components or analogues. Here, the self-assembly in water of two monodisperse lipid A derivatives based on simplified bacterial LPS structures is examined and compared to that of a native Escherichia coli LPS using small-angle X-ray scattering and cryogenic transmission electron microscopy. The critical aggregation concentration is obtained from fluorescence probe experiments, and conformation is probed using circular dichroism spectroscopy. The E. coli LPS is found to form wormlike micelles, whereas the synthetic analogues bearing six lipid chains and with four or two saccharide head groups (Kdo2-lipid A and monophosphoryl lipid A) self-assemble into nanosheets or vesicles, respectively. These observations are rationalized by considering the surfactant packing parameter.Description
Funding Information: This work was supported by an EPSRC Fellowship grant (reference EP/V053396/1) to I.W.H. We thank Diamond for the award of SAXS beamtime on B21 (ref. SM29895-1) and Nikul Khunti for assistance and the ESRF for beamtime on BM29 (ref. MX-2513) and Dihia Moussaoui for help. We acknowledge the use of facilities in the Chemical Analysis Facility (CAF) at the University of Reading. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.
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Castelletto, V, Seitsonen, J & Hamley, I W 2023, ' Effect of Glycosylation on Self-Assembly of Lipid A Lipopolysaccharides in Aqueous Solutions ', Langmuir, vol. 39, no. 24, pp. 8516-8522 . https://doi.org/10.1021/acs.langmuir.3c00828