Synthesis of Cellulose Hexanoate, Benzoate, and Mixed Esters: Exploring Their Potential as Enzyme Immobilization Platforms

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Authors

Date

2025-11

Department

Department of Bioproducts and Biosystems

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Mcode

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Language

en

Pages

16

Series

Macromolecular Bioscience, Volume 25, issue 11

Abstract

This study utilizes cellulose sourced from cotton linters to synthesize cellulose esters—hexanoate, benzoate, and mixed hexanoate-benzoate—with varying degrees of substitution (DS). These esters create electrospun mats that immobilize Pseudomonas fluorescens lipase (PFL), also in a configuration where an intermediate layer is added to a mat using an airbrush filled with PFL, covered by a third layer of electrospun mat. PFL-incorporated spheres are produced from cellulose ester solutions. DS, acyl chain length, and electrospinning parameters influence the morphology of the electrospun mat, which consists of nanofibers and ultrafine fibers. The PFL-incorporated mats show poor catalytic activity in resolving racemic (R,S)-2-chloro-1-phenylethanol, likely due to enzyme deactivation from high-voltage electrospinning. In contrast, mat-layered structures with PFL immobilized without voltage nearly doubled the conversion rate, although it was still lower than that of free enzymes. Spheres enhanced biocatalysis, achieving a 40% conversion rate with 94% enantiomeric purity while retaining 76% of their initial conversion rate in a subsequent reaction cycle. This research is the first to explore cellulose esters for the enzymatic immobilization of PFL to resolve a racemic mixture. The findings may enable PFL-incorporated structures in broader biocatalysis applications; the materials created may be tested to support the immobilization of other enzymes.

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Publisher Copyright: © 2025 The Author(s). Macromolecular Bioscience published by Wiley-VCH GmbH.

Keywords

cellulose ester spheres, cellulose esters, electrospun mats, enzyme immobilization, layered structure mats, racemic mixture resolution

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DOI

10.1002/mabi.202500221

Citation

Teixeira Polez, R, Voltarelli Ferracini, T, Cardoso de Paula, S F, Passos de Oliveira Santos, R, L.M. Porto, A & Frollini, E 2025, 'Synthesis of Cellulose Hexanoate, Benzoate, and Mixed Esters: Exploring Their Potential as Enzyme Immobilization Platforms', Macromolecular Bioscience, vol. 25, no. 11, e00221. https://doi.org/10.1002/mabi.202500221

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https://urn.fi/URN:NBN:fi:aalto-202601051064

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[article-cris] Kemian tekniikan korkeakoulu / CHEM