Layer-by-Layer Deposition of Low-Solid Nanochitin Emulgels Creates Porous Structures for High Cell Attachment and Proliferation
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2023-06-07
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en
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11
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ACS Applied Materials and Interfaces, Volume 15, issue 22, pp. 27316-27326
Abstract
Direct ink writing (DIW) is a customizable platform to engineer complex constructs from biobased colloids. However, the latter usually display strong interactions with water and lack interparticle connectivity, limiting one-step processing into hierarchically porous structures. We overcome such challenges by using low-solid emulgel inks stabilized by chitin nanofibrils (nanochitin, NCh). By using complementary characterization platforms, we reveal NCh structuring into spatially controlled three-dimensional (3D) materials that generate multiscale porosities defined by emulsion droplet size, ice templating, and DIW infill density. The extrusion variables, key in the development of surface and mechanical features of printed architectures, are comprehensively analyzed by using molecular dynamics and other simulation approaches. The obtained scaffolds are shown for their hierarchical porous structures, high areal density, and surface stiffness, which lead to excellent modulation of cell adhesion, proliferation, and differentiation, as tested with mouse dermal fibroblast expressing green fluorescent proteins.Description
| openaire: EC/H2020/788489/EU//BioELCell Funding Information: The authors acknowledge funding from the European Research Council under the European Union’s Horizon 2020 research and innovation program (ERC Advanced Grant Agreement No. 788489, “BioElCell”), the Canada Excellence Research Chair Program (CERC-2018-00006), and the Canada Foundation for Innovation (Project 38623). L.B. acknowledges the Natural Science Foundation of Heilongjiang Province (YQ2021C009). Y.Z. acknowledges the financial support from the China Scholarship Council (201873102). Imaging of cell work was performed at the Biomedicum Imaging Unit at the University of Helsinki, supported by the Helsinki Institute of Life Science (HiLIFE) and the Biocenter Finland. Antti Isomäki and Mikko Liljeström are acknowledged for their expert assistance in microscopy. Emilie Ressouche is acknowledged for assistance in interfacial tension tests and Tao Zou for assistance in stiffness tests using AFM. Z.W. and O.J.R. acknowledge the computational resources and services provided by Advanced Research Computing at the University of British Columbia. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.
Keywords
3D printing, cell proliferation, hierarchical porosity, nanochitin, Pickering emulsion
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Citation
Zhu, Y, Kankuri, E, Zhang, X, Wan, Z, Wang, X, Huan, S, Bai, L, Liu, S & Rojas, O J 2023, ' Layer-by-Layer Deposition of Low-Solid Nanochitin Emulgels Creates Porous Structures for High Cell Attachment and Proliferation ', ACS Applied Materials and Interfaces, vol. 15, no. 22, pp. 27316-27326 . https://doi.org/10.1021/acsami.3c03421