Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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en
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10
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ChemSusChem, Volume 12, issue 4, pp. 848-857
Abstract
Plant-derived carbohydrates are an abundant renewable resource. Transformation of carbohydrates into new products, including amine-functionalized building blocks for biomaterials applications, can lower reliance on fossil resources. Herein, biocatalytic production routes to amino carbohydrates, including oligosaccharides, are demonstrated. In each case, two-step biocatalysis was performed to functionalize d-galactose-containing carbohydrates by employing the galactose oxidase from Fusarium graminearum or a pyranose dehydrogenase from Agaricus bisporus followed by the ω-transaminase from Chromobacterium violaceum (Cvi-ω-TA). Formation of 6-amino-6-deoxy-d-galactose, 2-amino-2-deoxy-d-galactose, and 2-amino-2-deoxy-6-aldo-d-galactose was confirmed by mass spectrometry. The activity of Cvi-ω-TA was highest towards 6-aldo-d-galactose, for which the highest yield of 6-amino-6-deoxy-d-galactose (67 %) was achieved in reactions permitting simultaneous oxidation of d-galactose and transamination of the resulting 6-aldo-d-galactose.Description
| openaire: EC/H2020/648925/EU//BHIVE
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Aumala, V, Mollerup, F, Jurak, E, Blume, F, Karppi, J, Koistinen, A E, Schuiten, E, Voß, M, Bornscheuer, U, Deska, J & Master, E R 2019, 'Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades', ChemSusChem, vol. 12, no. 4, pp. 848-857. https://doi.org/10.1002/cssc.201802580