Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades

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Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2019-02-21
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Language
en
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ChemSusChem
Abstract
Plant-derived carbohydrates are an abundant renewable resource. Transformation of carbohydrates into new products, including amine-functionalized building blocks for biomaterials applications, can lower reliance on fossil resources. Herein, biocatalytic production routes to amino carbohydrates, including oligosaccharides, are demonstrated. In each case, two-step biocatalysis was performed to functionalize d-galactose-containing carbohydrates by employing the galactose oxidase from Fusarium graminearum or a pyranose dehydrogenase from Agaricus bisporus followed by the ω-transaminase from Chromobacterium violaceum (Cvi-ω-TA). Formation of 6-amino-6-deoxy-d-galactose, 2-amino-2-deoxy-d-galactose, and 2-amino-2-deoxy-6-aldo-d-galactose was confirmed by mass spectrometry. The activity of Cvi-ω-TA was highest towards 6-aldo-d-galactose, for which the highest yield of 6-amino-6-deoxy-d-galactose (67 %) was achieved in reactions permitting simultaneous oxidation of d-galactose and transamination of the resulting 6-aldo-d-galactose.
Description
| openaire: EC/H2020/648925/EU//BHIVE
Keywords
amination, biocatalysis, carbohydrates, domino reactions, enzymes
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Citation
Aumala, V, Mollerup, F, Jurak, E, Blume, F, Karppi, J, Koistinen, A E, Schuiten, E, Voß, M, Bornscheuer, U, Deska, J & Master, E R 2019, ' Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades ', ChemSusChem, vol. 12, no. 4, pp. 848-857 . https://doi.org/10.1002/cssc.201802580