Practical considerations for feature assignment in high-speed AFM of live cell membranes

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2022
Major/Subject
Mcode
Degree programme
Language
en
Pages
21
1-21
Series
Biophysics and Physicobiology, Volume 19
Abstract
High speed atomic force microscopy (HS-AFM) is, in principle, capable of yielding nanometer level detail about the surface of static structures. However, for highly dynamic samples HS-AFM may struggle with the correct feature assignment both within and between frames. Feature assignment in HS-AFM is dependent on (i) the intrinsic sampling rate, and (ii) the rate of internal redistribution of the sample. Whilst the first quantity (the sampling rate) is defined by the device parameters, the second quantity is frequently unknown, and is often the desired target of the measurement. This work examines how, even in the absence of gross cell morphological change, the rapid dynamics of living cell membranes, may impose an upper spatial limit to the frame-to-frame assignment of cell micro-topography and other related properties (such as local elasticity) whose motion may be described stochastically. Such a practical maximum may prove useful in the setup of HS-AFM experiments involving dynamic surfaces thereby facilitating selection of the most parsimonious relationship between observation size, image pixilation and sampling rates. To assist with performing the described calculations a graphical user interface-based software package called HS-AFM UGOKU is made freely available.
Description
Keywords
high-speed atomic force microscopy, experimental optimization, live cell membranes, dynamic surfaces, ATOMIC-FORCE MICROSCOPY, SINGLE-PARTICLE TRACKING, LATERAL DIFFUSION, HYDRODYNAMIC INTERACTIONS, PLASMA-MEMBRANE, SURFACE, PROTEINS, DYNAMICS, VISUALIZATION, DIVISION
Other note
Citation
Hall, D & Foster, A S 2022, ' Practical considerations for feature assignment in high-speed AFM of live cell membranes ', Biophysics and Physicobiology, vol. 19, 190016, pp. 1-21 . https://doi.org/10.2142/biophysico.bppb-v19.0016