Optically Controlled Construction of Three-Dimensional Protein Arrays

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2023-07-10
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Mcode
Degree programme
Language
en
Pages
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Angewandte Chemie - International Edition, articlenumber e202303880
Abstract
Protein crystallization is an important tool for structural biology and nanostructure preparation. Here, we report on kinetic pathway-dependent protein crystals that are controlled by light. Photo-responsive crystallites are obtained by complexing the model proteins with cationic azobenzene dyes. The crystalline state is readily switched to a dispersed phase under ultraviolet light and restored by subsequent visible-light illumination. The switching can be reversibly repeated for multiple cycles without noticeable structure deterioration. Importantly, the photo-treatment not only significantly increases the crystallinity, but creates crystallites at conditions where no ordered lattices are observed upon directly mixing the components. Further control over the azobenzene isomerization kinetics produces protein single crystals of up to ≈50 μm. This approach offers an intriguing method to fabricate metamaterials and study optically controlled crystallization.
Description
| openaire: EC/H2020/101002258/EU//ProCrystal Funding Information: This work has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (Grant Agreement No. 101002258). We acknowledge the funding from the Jane and Aatos Erkko Foundation, the Magnus Ehrnrooth Foundation, Tampere University Graduate School, the National Nature Science Foundation of China (No. 22277018), the Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholar (LR23B030001), and Wenzhou Institute, University of Chinese Academy of Sciences (No. WIUCASQD2020015, No. WIUCASQD2022006, No. WIUCASQD2021048). We acknowledge the provision of facilities and technical support by Aalto University Bioeconomy Facilities and OtaNano‐Nanomicroscopy Center (Aalto‐NMC). The authors thank Jani Seitsonen from the Department of Applied Physics, Aalto University, for the support in cryo‐TEM measurements. This work was carried out under the Academy of Finland's Centers of Excellence Programme, Life Inspired Hybrid Materials (LIBER) Center of Excellence (2022–2029), and the Flagship Programme PREIN (No. 320165). Publisher Copyright: © 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
Keywords
Azobenzene, Crystallization, Kinetic Pathway, Photo Responsiveness, Protein Cage
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Citation
Liu , Q , Zhou , Y , Shaukat , A , Meng , Z , Kyllönen , D , Seitz , I , Langerreiter , D , Kuntze , K , Priimagi , A , Zheng , L & Kostiainen , M A 2023 , ' Optically Controlled Construction of Three-Dimensional Protein Arrays ' , Angewandte Chemie - International Edition , vol. 62 , no. 28 , e202303880 . https://doi.org/10.1002/anie.202303880