The effects of dietary energy level during periparturient period on hepatic gene expressions in dairy cows

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.advisorElo, Kari
dc.contributor.advisorHoti, Fabian J.
dc.contributor.authorGulzar, Zohaib
dc.contributor.departmentPerustieteiden korkeakoulufi
dc.contributor.schoolPerustieteiden korkeakoulufi
dc.contributor.schoolSchool of Scienceen
dc.contributor.supervisorRousu, Juho
dc.date.accessioned2020-12-28T15:11:31Z
dc.date.available2020-12-28T15:11:31Z
dc.date.issued2013
dc.description.abstractPeri-parturient diseases in dairy cows due to metabolic disorders are wel1 known in the dairy industry. Metabolic disorders can also lead to reduced fertility. The main components affecting these disorders include severe negative energy balance (SNEB) and insulin resistance. These are mainly due to the increased demand of energy for a few weeks during the postpartum period. Recent studies show that this can be medicated by controlling the feed intake on dairy cows. This thesis describes the effects of dietary energy level during the peri-parturient period on hepatic gene expressions in dairy cows. The dietary energy level groups involve the ad libitum, which obtained 150% of the total requirement and the restricted energy, which obtained 100 % of the total energy requirement. The main purpose is to evaluate the association between these two dietary groups of cows and their gene expressions. In this experiment, eleven multiparous Ayrshire cows were randomly selected and total of 32 microchips obtained for 3 time points, 8 days before calving, 1 day after calving, and 9 days after calving. This thesis will analyse the expression of bovine Affymetrix arrays, comparing the control group with the experiment at different time points. Furthermore, this study includes analysis of differentially expressed genes within the groups before and after calving and discusses their relation to feed intake and regulatory groups. Functional pathways analysis of Differentially Expressed Genes (DEG) shows that the genes were related to the networks involved in lipid metabolism, cell signalling, molecular transport, and small molecule biochemistry.en
dc.format.extent59
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/100873
dc.identifier.urnURN:NBN:fi:aalto-2020122859704
dc.language.isoenen
dc.programme.majorInformaatiotekniikkafi
dc.programme.mcodeT-61fi
dc.rights.accesslevelclosedAccess
dc.subject.keywordmicro arrayen
dc.subject.keywordBovineen
dc.subject.keywordRen
dc.subject.keywordgenesen
dc.subject.keywordaffymetrixen
dc.subject.keywordgene networken
dc.titleThe effects of dietary energy level during periparturient period on hepatic gene expressions in dairy cowsen
dc.type.okmG2 Pro gradu, diplomityö
dc.type.ontasotMaster's thesisen
dc.type.ontasotPro gradu -tutkielmafi
dc.type.publicationmasterThesis
local.aalto.digiauthask
local.aalto.digifolderAalto_01356
local.aalto.idinssi47259
local.aalto.openaccessno
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