Bioinspired Nanochitin-Based Porous Constructs for Light-Driven Whole-Cell Biotransformations

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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en

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12

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Advanced Materials, Volume 37, issue 22

Abstract

Solid-state photosynthetic cell factories (SSPCFs) are a new production concept that leverages the innate photosynthetic abilities of microbes to drive the production of valuable chemicals. It addresses practical challenges such as high energy and water demand and improper light distribution associated with suspension-based culturing; however, these systems often face significant challenges related to mass transfer. The approach focuses on overcoming these limitations by carefully engineering the microstructure of the immobilization matrix through freeze-induced assembly of nanochitin building blocks. The use of nanochitins with optimized size distribution enabled the formation of macropores with lamellar spatial organization, which significantly improves light transmittance and distribution, crucial for maximizing the efficiency of photosynthetic reactions. The biomimetic crosslinking strategy, leveraging specific interactions between polyphosphate anions and primary amine groups featured on chitin fibers, produced mechanically robust and wet-resilient cryogels that maintained their functionality under operational conditions. Various model biotransformation reactions leading to value-added chemicals are performed in chitin-based matrix. It demonstrates superior or comparable performance to existing state-of-the-art matrices and suspension-based systems. The findings suggest that chitin-based cryogel approach holds significant promise for advancing the development of solid-state photosynthetic cell factories, offering a scalable solution to improve the efficiency and productivity of light-driven biotransformation.

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| openaire: EC/H2020/899576/EU//FuturoLEAF

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Arumughan, V, Medipally, H, Torris, A, Levä, T, Grimm, H C, Tammelin, T, Kourist, R & Kontturi, E 2025, 'Bioinspired Nanochitin-Based Porous Constructs for Light-Driven Whole-Cell Biotransformations', Advanced Materials, vol. 37, no. 22, 2413058. https://doi.org/10.1002/adma.202413058