Coacervation of resilin fusion proteins containing terminal functionalities

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2018-11-01
Major/Subject
Mcode
Degree programme
Language
en
Pages
7
590-596
Series
Colloids and Surfaces B: Biointerfaces, Volume 171
Abstract
Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.
Description
Keywords
Resilin, Self-assembly, Coacervation, Selective adhesion, Tomography, TROPOELASTIN, HYDROPHOBINS, CELLULOSE, REC1-RESILIN, GRAPHENE
Other note
Citation
Fang, W, Nonappa, Vitikainen, M, Mohammadi, P, Koskela, S, Soikkeli, M, Westerholm-Parvinen, A, Landowski, C P, Penttilä, M, Linder, M B & Laaksonen, P 2018, ' Coacervation of resilin fusion proteins containing terminal functionalities ', Colloids and Surfaces B: Biointerfaces, vol. 171, pp. 590-596 . https://doi.org/10.1016/j.colsurfb.2018.07.048