TRPV6 calcium channel directs homeostasis of the mammary epithelial sheets and controls epithelial mesenchymal transition

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorKärki, Tyttien_US
dc.contributor.authorRajakylä, Eeva Kaisaen_US
dc.contributor.authorAcheva, Annaen_US
dc.contributor.authorTojkander, Sarien_US
dc.contributor.departmentDepartment of Applied Physicsen
dc.contributor.groupauthorActive Matteren
dc.contributor.organizationUniversity of Helsinkien_US
dc.date.accessioned2020-10-02T06:25:05Z
dc.date.available2020-10-02T06:25:05Z
dc.date.issued2020-09-07en_US
dc.description.abstractEpithelial integrity is lost upon cancer progression as cancer cells detach from the primary tumor site and start to invade to the surrounding tissues. Invasive cancers of epithelial origin often express altered levels of TRP-family cation channels. Upregulation of TRPV6 Ca2+-channel has been associated with a number of human malignancies and its high expression in breast cancer has been linked to both proliferation and invasive disease. The mechanisms behind the potential of TRPV6 to induce invasive progression have, however, not been well elucidated. Here we show that TRPV6 is connected to both E-cadherin-based adherens junctions and intracellular cytoskeletal structures. Loss of TRPV6 from normal mammary epithelial cells led to disruption of epithelial integrity and abnormal 3D-mammo sphere morphology. Furthermore, expression level of TRPV6 was tightly linked to the levels of common EMT markers, suggesting that TRPV6 may have a role in the mesenchymal invasion of breast cancer cells. Thus, either too low or too high TRPV6 levels compromise homeostasis of the mammary epithelial sheets and may promote the progression of pathophysiological conditions.en
dc.description.versionPeer revieweden
dc.format.extent15
dc.format.extent1-15
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationKärki, T, Rajakylä, E K, Acheva, A & Tojkander, S 2020, ' TRPV6 calcium channel directs homeostasis of the mammary epithelial sheets and controls epithelial mesenchymal transition ', Scientific Reports, vol. 10, no. 1, 14683, pp. 1-15 . https://doi.org/10.1038/s41598-020-71645-zen
dc.identifier.doi10.1038/s41598-020-71645-zen_US
dc.identifier.issn2045-2322
dc.identifier.otherPURE UUID: ce3576d9-8d1a-4a50-8e80-e3e7dcec43b1en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/ce3576d9-8d1a-4a50-8e80-e3e7dcec43b1en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85090295865&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/51811047/K_rki_TRPV6.s41598_020_71645_z_1.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/46821
dc.identifier.urnURN:NBN:fi:aalto-202010025786
dc.language.isoenen
dc.publisherNature Publishing Group
dc.relation.ispartofseriesScientific Reportsen
dc.relation.ispartofseriesVolume 10, issue 1en
dc.rightsopenAccessen
dc.titleTRPV6 calcium channel directs homeostasis of the mammary epithelial sheets and controls epithelial mesenchymal transitionen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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