Simultaneous Detection of Morphine and Codeine in the Presence of Ascorbic Acid and Uric Acid and in Human Plasma at Nafion Single-Walled Carbon Nanotube Thin-Film Electrode

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorWester, Niklasen_US
dc.contributor.authorMynttinen, Elsien_US
dc.contributor.authorEtula, Jarkkoen_US
dc.contributor.authorLilius, Tuomasen_US
dc.contributor.authorKalso, Eijaen_US
dc.contributor.authorKauppinen, Esko I.en_US
dc.contributor.authorLaurila, Tomien_US
dc.contributor.authorKoskinen, Jarien_US
dc.contributor.departmentDepartment of Chemistry and Materials Scienceen
dc.contributor.departmentDepartment of Electrical Engineering and Automationen
dc.contributor.departmentDepartment of Applied Physicsen
dc.contributor.groupauthorPhysical Characteristics of Surfaces and Interfacesen
dc.contributor.groupauthorMicrosystems Technologyen
dc.contributor.groupauthorNanoMaterialsen
dc.contributor.organizationUniversity of Helsinkien_US
dc.date.accessioned2019-11-07T12:09:13Z
dc.date.available2019-11-07T12:09:13Z
dc.date.issued2019-01-01en_US
dc.description.abstractIn clinical settings, the dosing and differential diagnosis of the poisoning of morphine (MO) and codeine (CO) is challenging due to interindividual variations in metabolism. However, direct electrochemical detection of these analytes from biological matrices is inherently challenging due to interference from large concentrations of anions, such as ascorbic acid (AA) and uric acid (UA), as well as fouling of the electrode by proteins. In this work, a disposable Nafion-coated single-walled carbon nanotube network (SWCNT) electrode was developed. We show facile electron transfer and efficient charge separation between the interfering anions and positively charged MO and CO, as well as significantly reduced matrix effect in human plasma. The Nafion coating alters the voltammetric response of MO and CO, enabling simultaneous detection. With this SWCNT/Nafion electrode, two linear ranges of 0.05-1 and 1-10 μM were found for MO and one linear range of 0.1-50 μM for CO. Moreover, the selective and simultaneous detection of MO and CO was achieved in large excess of AA and UA, as well as, for the first time, in unprocessed human plasma. The favorable properties of this electrode enabled measurements in plasma with only mild dilution and without the precipitation of proteins.en
dc.description.versionPeer revieweden
dc.format.extent9
dc.format.extent17726-17734
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationWester, N, Mynttinen, E, Etula, J, Lilius, T, Kalso, E, Kauppinen, E I, Laurila, T & Koskinen, J 2019, ' Simultaneous Detection of Morphine and Codeine in the Presence of Ascorbic Acid and Uric Acid and in Human Plasma at Nafion Single-Walled Carbon Nanotube Thin-Film Electrode ', ACS Omega, pp. 17726-17734 . https://doi.org/10.1021/acsomega.9b02147en
dc.identifier.doi10.1021/acsomega.9b02147en_US
dc.identifier.issn2470-1343
dc.identifier.otherPURE UUID: e9e9571c-b6fe-4735-8f83-a326923fe40cen_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/e9e9571c-b6fe-4735-8f83-a326923fe40cen_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85073872528&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/38373334/acsomega.9b02147.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/41179
dc.identifier.urnURN:NBN:fi:aalto-201911076184
dc.language.isoenen
dc.publisherAMERICAN CHEMICAL SOCIETY
dc.relation.ispartofseriesACS Omegaen
dc.rightsopenAccessen
dc.titleSimultaneous Detection of Morphine and Codeine in the Presence of Ascorbic Acid and Uric Acid and in Human Plasma at Nafion Single-Walled Carbon Nanotube Thin-Film Electrodeen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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