Infrared photo-induced force microscopy unveils nanoscale features of Norway spruce fibre wall

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2021-07
Major/Subject
Mcode
Degree programme
Language
en
Pages
15
7295-7309
Series
Cellulose, Volume 28, issue 11
Abstract
Infrared photo-induced force microscopy (IR PiFM) was applied for imaging ultrathin sections of Norway spruce (Picea abies) at 800–1885 cm−1 with varying scanning steps from 0.6 to 30 nm. Cell wall sublayers were visualized in the low-resolution mode based on differences in their chemical composition. The spectra from the individual sublayers demonstrated differences in the orientation of cellulose elementary fibrils (EFs) and in the content and structure of lignin. The high-resolution images revealed 5–20 nm wide lignin-free areas in the S1 layer. Full spectra collected from a non-lignified spot and at a short distance apart from it verified an abrupt change in the lignin content and the presence of tangentially oriented EFs. Line scans across the lignin-free areas corresponded to a spatial resolution of ≤ 5 nm. The ability of IR PiFM to resolve structures based on their chemical composition differentiates it from transmission electron microscopy that can reach a similar spatial resolution in imaging ultrathin wood sections. In comparison with Raman imaging, IR PiFM can acquire chemical images with ≥ 50 times higher spatial resolution. IR PiFM is also a surface-sensitive technique that is important for reaching the high spatial resolution in anisotropic samples like the cell wall. All these features make IR PiFM a highly promising technique for analyzing the recalcitrant nature of lignocellulosic biomass for its conversion into various materials and chemicals. Graphic abstract: [Figure not available: see fulltext.]
Description
Funding Information: Open access funding provided by Aalto University. We thankfully acknowledge the Academy of Finland (grant number 290324) for financial support. Publisher Copyright: © 2021, The Author(s).
Keywords
Cellulose elementary fibril, Chemical imaging, Infrared spectroscopy, Lignin, Photo-induced force microscopy, Plant cell wall, Spruce wood
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Citation
Kesari , K K , O’Reilly , P , Seitsonen , J , Ruokolainen , J & Vuorinen , T 2021 , ' Infrared photo-induced force microscopy unveils nanoscale features of Norway spruce fibre wall ' , Cellulose , vol. 28 , no. 11 , pp. 7295-7309 . https://doi.org/10.1007/s10570-021-04006-2