Self-Assembling Protein-Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Binding

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dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorLiu, Yingyingen_US
dc.contributor.authorNevanen, Tarja K.en_US
dc.contributor.authorPaananen, Arjaen_US
dc.contributor.authorKempe, Kristianen_US
dc.contributor.authorWilson, Paulen_US
dc.contributor.authorJohansson, Leena Siskoen_US
dc.contributor.authorJoensuu, Jussi J.en_US
dc.contributor.authorLinder, Markus B.en_US
dc.contributor.authorHaddleton, David M.en_US
dc.contributor.authorMilani, Robertoen_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.groupauthorBio-based Colloids and Materialsen
dc.contributor.groupauthorBiomolecular Materialsen
dc.contributor.organizationVTT Technical Research Centre of Finlanden_US
dc.contributor.organizationUniversity of Warwicken_US
dc.date.accessioned2019-05-06T09:23:47Z
dc.date.available2019-05-06T09:23:47Z
dc.date.issued2019-01-23en_US
dc.description.abstractA new method is demonstrated for preparing antifouling and low nonspecific adsorption surfaces on poorly reactive hydrophobic substrates, without the need for energy-intensive or environmentally aggressive pretreatments. The surface-active protein hydrophobin was covalently modified with a controlled radical polymerization initiator and allowed to self-assemble as a monolayer on hydrophobic surfaces, followed by the preparation of antifouling surfaces by Cu(0)-mediated living radical polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA) performed in situ. By taking advantage of hydrophobins to achieve at the same time the immobilization of protein A, this approach allowed to prepare surfaces for IgG1 binding featuring greatly reduced nonspecific adsorption. The success of the surface modification strategy was investigated by contact angle, XPS, and AFM characterization, while the antifouling performance and the reduction of nonspecific binding were confirmed by QCM-D measurements.en
dc.description.versionPeer revieweden
dc.format.extent10
dc.format.extent3599-3608
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationLiu, Y, Nevanen, T K, Paananen, A, Kempe, K, Wilson, P, Johansson, L S, Joensuu, J J, Linder, M B, Haddleton, D M & Milani, R 2019, ' Self-Assembling Protein-Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Binding ', ACS Applied Materials and Interfaces, vol. 11, no. 3, pp. 3599-3608 . https://doi.org/10.1021/acsami.8b19968en
dc.identifier.doi10.1021/acsami.8b19968en_US
dc.identifier.issn1944-8244
dc.identifier.issn1944-8252
dc.identifier.otherPURE UUID: d476a595-c59e-4fb3-9680-5c5b85b5d5d5en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/d476a595-c59e-4fb3-9680-5c5b85b5d5d5en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85060464761&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/32829700/CHEM_Liu_et_al_Self_Assembling_Protein_Polymer_2019_acs_Applied_Materials_and_Interfaces.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/37775
dc.identifier.urnURN:NBN:fi:aalto-201905062893
dc.language.isoenen
dc.publisherAMER CHEMICAL SOC
dc.relation.ispartofseriesACS Applied Materials and Interfacesen
dc.relation.ispartofseriesVolume 11, issue 3en
dc.rightsopenAccessen
dc.subject.keywordantifouling surfacesen_US
dc.subject.keywordbiosensorsen_US
dc.subject.keywordhydrophobinen_US
dc.subject.keywordnonspecific bindingen_US
dc.subject.keywordsurface-initiated living radical polymerizationen_US
dc.titleSelf-Assembling Protein-Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Bindingen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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[article-cris] Kemian tekniikan korkeakoulu / CHEM