Structuring of Nanocelluloses in 3-D Functional Materials

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School of Chemical Technology | Doctoral thesis (article-based) | Defence date: 2022-01-27
Degree programme
66 + app. 116
Aalto University publication series DOCTORAL THESES, 4/2022
This thesis investigates fundamental and practical aspects of the materials and methods used to assemble 3D structures from bio-based materials using mono- to multi-component systems through bottom-up and other processes. Fused Deposition Molding (FDM) and Direct Ink Writing (DIW) were used to fabricate structures with controlled geometries and properties. Nanocellulose from both microorganisms and wood featured promising biocompatibility and was investigated as primary subjects for the studies. We discuss the rheological requirements to process hydrogels by direct ink writing and address the effect of compositions and water interactions in the swelling of 3D-printed materials. The essential factors associated with cellular activities in biomedical applications were considered. The shear-thinning behavior of nanocellulose hydrogels facilitated the printability of the inks into defined shapes, which were investigated by using a wide range of needle sizes, lengths, and profiles. We discuss the effect of printing parameters and post-processing techniques on structural fidelity and properties. The nanocellulose-based mono and multi-component functional structures presented the advantages of inexpensive and fast production, dimensional retention and stability, ease of drying and rewetting process, thus facilitating packaging, transportation, and material sterilization while displaying excellent compatibility with cells. Material characterizations (for example, morphology, microstructure, mechanical performance, shrinkage, swelling, and degradation) were studied to define suitable applications for the developed structures. Our findings in this thesis are expected to facilitate future work to address standing challenges in constructing 3-dimensional bio-based materials.
Defence is held on 27.1.2022 18:00 – 21:00
Supervising professor
Rojas, Orlando, Prof., Aalto University, Department of Bioproducts and Biosystems, Finland
Thesis advisor
Rojas, Orlando, Prof., Aalto University, Finland
Huan, Siqi, Dr., Aalto University, Finland
Tardy, Blaise L., Dr., Aalto University, Finland
nanocellulose, biopolymer, three-dimensional, additive manufacturing
Other note
  • [Publication 1]: Ajdary, Rubina; Abidnejad, Roozbeh; Lehtonen, Janika; Kuula, Jani; Raussi-Lehto, Eija; Kankuri, Esko; Tardy, Blaise; Rojas, Orlando J. Bacterial aerotaxis as a route toward cellulose biomaterials and auxetic supporting im- plants. Submitted to the Carbohydrate Polymers (2021)
  • [Publication 2]: Ajdary, Rubina; Huan, Siqi; Zanjanizadeh Ezazi, Nazanin; Xiang, Wenchao; Grande, Rafael; Santos, Hélder A; Rojas, Orlando J. (2019) Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D- printed scaffolds. Biomacromolecules, 20 (7), 2770–2778.
    Full text in Acris/Aaltodoc:
    DOI: 10.1021/acs.biomac.9b00527 View at publisher
  • [Publication 3]: Baniasadi, Hossein; Ajdary, Rubina; Trifol, Jon; Rojas, Orlando J; Seppälä, Jukka. (2021) Direct ink writing of aloe vera/cellulose nanofibrils biohydrogels. Carbohydrate Polymers, 266, 118114.
    Full text in Acris/Aaltodoc:
    DOI: 10.1016/j.carbpol.2021.118114 View at publisher
  • [Publication 4]: Ajdary, Rubina; Reyes, Guillermo; Kuula, Jani; Raussi-Lehto, Eija; Mikkola, Tomi S.; Kankuri, Esko; Rojas, Orlando J. Direct ink writing of biocompatible nanocellulose and chitosan hydrogels for implant mesh matrices. Accepted in the ACS Polymers Au (2021).
    Full text in Acris/Aaltodoc:
    DOI: 10.1021/acspolymersau.1c00045 View at publisher
  • [Publication 5]: Ajdary, Rubina; Zanjanizadeh Ezazi, Nazanin; Rebelo Correia, Alexandra Maria; Kemell, Marianna; Huan, Siqi; Ruskoaho, Heikki; Hirvonen, Jouni; Santos, Hélder A; Rojas, Orlando J. (2020) Multifunctional 3D-printed patches for long-term drug release therapies after myocardial infarction. Advanced Functional Materials, 30, 2003440.
    Full text in Acris/Aaltodoc:
    DOI: 10.1002/adfm.202003440 View at publisher