Ultrasound-Enhanced Fine-Needle Biopsy Improves Yield in Human Epithelial and Lymphoid Tissue

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2024-08
Major/Subject
Mcode
Degree programme
Language
en
Pages
8
Series
ULTRASOUND IN MEDICINE AND BIOLOGY, Volume 50, issue 8, pp. 1247-1254
Abstract
Objective: Needle biopsy is a common technique used to obtain cell and tissue samples for diagnostics. Currently, two biopsy methods are widely used: (i) fine-needle aspiration biopsy (FNAB) and (ii) core needle biopsy (CNB). However, these methods have limitations. Recently, we developed ultrasound-enhanced fine-needle aspiration biopsy (USeFNAB), which employs a needle that flexurally oscillates at an ultrasonic frequency of ∼32 kHz. The needle motion contributes to increased tissue collection while preserving cells and tissue constructs for pathological assessment. Previously, USeFNAB has been investigated only in ex vivo animal tissue. The present study was aimed at determining the feasibility of using USeFNAB in human epithelial and lymphoid tissue. Methods: Needle biopsy samples were acquired using FNAB, CNB and USeFNAB on ex vivo human tonsils (N = 10). The tissue yield and quality were quantified by weight measurement and blinded pathologists’ assessments. The biopsy methods were then compared. Results: The results revealed sample mass increases of, on average, 2.3- and 5.4-fold with USeFNAB compared with the state-of-the-art FNAB and CNB, respectively. The quality of tissue fragments collected by USeFNAB was equivalent to that collected by the state-of-the-art methods in terms of morphology and immunohistochemical stainings made from cell blocks as judged by pathologists. Conclusion: Our study indicates that USeFNAB is a promising method that could improve tissue yield to ensure sufficient material for ancillary histochemical and molecular studies for diagnostic pathology, thereby potentially increasing diagnostic accuracy.
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Publisher Copyright: © 2024 The Authors
Keywords
Biopsy, Core needle biopsy, Fine-needle aspiration, Flexural actuation, Medical needle, Tonsil, Ultrasound
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Citation
Le Bourlout, Y, Rehell, M, Kelppe, J, Rautava, J, Perra, E, Rantanen, J, Ehnholm, G, Hayward, N, Nyman, K, Pritzker, K P H, Tarkkanen, J, Atula, T, Aro, K & Nieminen, H J 2024, ' Ultrasound-Enhanced Fine-Needle Biopsy Improves Yield in Human Epithelial and Lymphoid Tissue ', ULTRASOUND IN MEDICINE AND BIOLOGY, vol. 50, no. 8, pp. 1247-1254 . https://doi.org/10.1016/j.ultrasmedbio.2024.04.015