Polarized THG Microscopy Identifies Compositionally Different Lipid Droplets in Mammalian Cells

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dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorBautista, G.
dc.contributor.authorPfisterer, Simon G.
dc.contributor.authorHuttunen, M.J.
dc.contributor.authorKanerva, K.
dc.contributor.authorIkonen, Elina
dc.contributor.authorKauranen, Martti
dc.contributor.departmentDepartment of Applied Physicsen
dc.date.accessioned2017-05-11T08:40:21Z
dc.date.available2017-05-11T08:40:21Z
dc.date.issued2014
dc.description.abstractCells store excess lipids as two major compounds, triacylglycerols (TAGs) and cholesteryl esters (CEs), inside lipid droplets (LDs). The degree of lipid ordering is considered to play a major role in the mobility and enzymatic processing of lipids in LDs. Here, we provide evidence that polarized third-harmonic generation (THG) microscopy distinguishes between native TAG- and CE-enriched LDs in cells due to the different ordering of the two lipid species. We first demonstrate that the responses from synthetic TAG- and CE-enriched LDs using THG microscopy with linear and circular polarizations differ according to their different intrinsic ordering. We then employ simulations to dissect how polarization effects influence the THG from an isotropic LD. Finally, we induce TAG- and CE-enriched LDs in murine macrophages and demonstrate that polarized THG responses increase in a nonlinear fashion with increasing CE/TAG ratio. This suggests that with an increasing CE content, there is a rather sharp transition toward increased LD ordering. Our results demonstrate that polarized THG microscopy enables label-free quantitative analysis of LD ordering and discriminates between compositionally different LDs in intact mammalian cells.en
dc.description.versionPeer revieweden
dc.format.extent2230-2236
dc.format.mimetypeapplication/pdf
dc.identifier.citationBautista , G , Pfisterer , S G , Huttunen , M J , Kanerva , K , Ikonen , E & Kauranen , M 2014 , ' Polarized THG Microscopy Identifies Compositionally Different Lipid Droplets in Mammalian Cells ' , Biophysical Journal , vol. 107 , pp. 2230-2236 . https://doi.org/10.1016/j.bpj.2014.10.009en
dc.identifier.doi10.1016/j.bpj.2014.10.009
dc.identifier.issn0006-3495
dc.identifier.issn1542-0086
dc.identifier.otherPURE UUID: 9623d35b-d01d-4fa3-a44b-849c4b42425f
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/9623d35b-d01d-4fa3-a44b-849c4b42425f
dc.identifier.otherPURE LINK: http://www.cell.com/biophysj/abstract/S0006-3495%2814%2901060-1
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/11717101/PIIS0006349514010601.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/25710
dc.identifier.urnURN:NBN:fi:aalto-201705114094
dc.language.isoenen
dc.relation.ispartofseriesBIOPHYSICAL JOURNALen
dc.relation.ispartofseriesVolume 107en
dc.rightsopenAccessen
dc.subject.keywordlipid
dc.subject.keywordmicroscopy
dc.subject.keywordpolarization
dc.titlePolarized THG Microscopy Identifies Compositionally Different Lipid Droplets in Mammalian Cellsen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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