In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorYuryev, Mikhail
dc.contributor.authorAndriichuk, Liliia
dc.contributor.authorLeiwe, Marcus
dc.contributor.authorJokinen, Ville
dc.contributor.authorCarabalona, Aurelie
dc.contributor.authorRivera, Claudio
dc.contributor.departmentUniversity of Helsinki
dc.contributor.departmentRIKEN
dc.contributor.departmentDepartment of Chemistry and Materials Science
dc.contributor.departmentInstitut national de la santé et de la recherche médicale
dc.date.accessioned2018-12-10T10:19:44Z
dc.date.available2018-12-10T10:19:44Z
dc.date.issued2018-10-30
dc.description.abstractPrior to sensory experience spontaneous activity appears to play a fundamental role in the correct formation of prominent functional features of different cortical regions. The use of anaesthesia during pregnancy such as ketamine is largely considered to negatively affect neuronal development by interfering with synaptic transmission. Interestingly, the characteristics of spontaneous activity as well as the acute functional effects of maternal anaesthesia remain largely untested in the embryonic cortex in vivo. In the present work, we performed in vivo imaging of spontaneous calcium activity and cell motility in the marginal zone of the cortex of E14-15 embryos connected to the mother. We made use of a preparation where the blood circulation from the mother through the umbilical cord is preserved and fluctuations in intracellular calcium in the embryonic frontal cortex are acquired using two-photon imaging. We found that spontaneous transients were either sporadic or correlated in clusters of neuronal ensembles at this age. These events were not sensitive to maternal isoflurane anaesthesia but were strongly inhibited by acute in situ or maternal application of low concentration of the anaesthetic ketamine (a non-competitive antagonist of NMDA receptors). Moreover, simultaneous imaging of cell motility revealed a correlated strong sensitivity to ketamine. These results show that anaesthetic compounds can differ significantly in their impact on spontaneous early cortical activity as well as motility of cells in the marginal zone. The effects found in this study may be relevant in the etiology of heightened vulnerability to cerebral dysfunction associated with the use of ketamine during pregnancy.en
dc.description.versionPeer revieweden
dc.format.extent1
dc.format.mimetypeapplication/pdf
dc.identifier.citationYuryev , M , Andriichuk , L , Leiwe , M , Jokinen , V , Carabalona , A & Rivera , C 2018 , ' In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity ' , Scientific Reports , vol. 8 , no. 1 . https://doi.org/10.1038/s41598-018-34410-xen
dc.identifier.doi10.1038/s41598-018-34410-x
dc.identifier.issn2045-2322
dc.identifier.otherPURE UUID: 68cf1eb3-5aa0-4192-a9c7-e5fae88540c5
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/68cf1eb3-5aa0-4192-a9c7-e5fae88540c5
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85055618563&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/29548502/CHEM_Yuryev_et_al_In_vivo_two_photon_2018_Scientific_Reports.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/35087
dc.identifier.urnURN:NBN:fi:aalto-201812106102
dc.language.isoenen
dc.relation.ispartofseriesScientific Reportsen
dc.relation.ispartofseriesVolume 8, issue 1en
dc.rightsopenAccessen
dc.titleIn vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activityen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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