Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorKujala, Johanna
dc.contributor.authorWester, Niklas
dc.contributor.authorLohela, Terhi J.
dc.contributor.authorKurkela, Mika
dc.contributor.authorBackman, Janne T.
dc.contributor.authorMikladal, Björn
dc.contributor.authorLaurila, Tomi
dc.contributor.authorKoskinen, Jari
dc.contributor.authorLilius, Tuomas O.
dc.contributor.authorKalso, Eija A.
dc.contributor.departmentHelsinki University Central Hospital
dc.contributor.departmentDepartment of Electrical Engineering and Automation
dc.contributor.departmentUniversity of Helsinki
dc.contributor.departmentCanatu Oy
dc.contributor.departmentDepartment of Chemistry and Materials Science
dc.contributor.departmentDepartment of Electrical Engineering and Automationen
dc.contributor.departmentDepartment of Chemistry and Materials Scienceen
dc.date.accessioned2023-08-30T04:20:03Z
dc.date.available2023-08-30T04:20:03Z
dc.date.issued2023-09
dc.descriptionThe authors would like to thank A/Prof. Mark Chatfield, University of Queensland, Australia, for his assistance with Stata code for Bland–Altman and Passing-Bablok analysis; and Laura Tervala, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland; and Meeri Alanappa, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland, for their skilful assistance.
dc.description.abstractAims: Measuring venous plasma paracetamol concentrations is time- and resource-consuming. We aimed to validate a novel electrochemical point-of-care (POC) assay for rapid paracetamol concentration determinations. Methods: Twelve healthy volunteers received 1 g oral paracetamol, and its concentrations were analysed 10 times over 12 h for capillary whole blood (POC), venous plasma (high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS)), and dried capillary blood (HPLC-MS/MS). Results: At concentrations >30 μM, POC showed upward biases of 20% (95% limits of agreement [LOA] −22 to 62) and 7% (95% LOA −23 to 38) compared with venous plasma and capillary blood HPLC-MS/MS, respectively. There were no significant differences between mean concentrations for the paracetamol elimination phase. Conclusions: Upward biases in POC compared with venous plasma HPLC-MS/MS were likely due to higher paracetamol concentrations in capillary blood than in venous plasma and to faulty individual sensors. The novel POC method is a promising tool for paracetamol concentration analysis.en
dc.description.versionPeer revieweden
dc.format.extent6
dc.format.extent2933-2938
dc.format.mimetypeapplication/pdf
dc.identifier.citationKujala , J , Wester , N , Lohela , T J , Kurkela , M , Backman , J T , Mikladal , B , Laurila , T , Koskinen , J , Lilius , T O & Kalso , E A 2023 , ' Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples ' , British Journal of Clinical Pharmacology , vol. 89 , no. 9 , pp. 2933-2938 . https://doi.org/10.1111/bcp.15794en
dc.identifier.doi10.1111/bcp.15794
dc.identifier.issn0306-5251
dc.identifier.issn1365-2125
dc.identifier.otherPURE UUID: 24d368fb-59e5-4763-8113-499649e76968
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/24d368fb-59e5-4763-8113-499649e76968
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85161489597&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/119687465/CHEM_Kujala_et_al_Introduction_of_an_electrochemical_2023_Br_J_Clin_Pharmacol.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/122964
dc.identifier.urnURN:NBN:fi:aalto-202308305304
dc.language.isoenen
dc.publisherWiley-Blackwell
dc.relation.ispartofseriesBritish Journal of Clinical Pharmacologyen
dc.relation.ispartofseriesVolume 89, issue 9en
dc.rightsopenAccessen
dc.subject.keywordacetaminophen
dc.subject.keywordcapillary
dc.subject.keyworddiagnostics
dc.subject.keywordintoxication
dc.subject.keywordparacetamol
dc.subject.keywordpoint-of-care
dc.subject.keywordSWCNT
dc.titleIntroduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samplesen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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