Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2023-09
Major/Subject
Mcode
Degree programme
Language
en
Pages
6
2933-2938
Series
British Journal of Clinical Pharmacology, Volume 89, issue 9
Abstract
Aims: Measuring venous plasma paracetamol concentrations is time- and resource-consuming. We aimed to validate a novel electrochemical point-of-care (POC) assay for rapid paracetamol concentration determinations. Methods: Twelve healthy volunteers received 1 g oral paracetamol, and its concentrations were analysed 10 times over 12 h for capillary whole blood (POC), venous plasma (high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS)), and dried capillary blood (HPLC-MS/MS). Results: At concentrations >30 μM, POC showed upward biases of 20% (95% limits of agreement [LOA] −22 to 62) and 7% (95% LOA −23 to 38) compared with venous plasma and capillary blood HPLC-MS/MS, respectively. There were no significant differences between mean concentrations for the paracetamol elimination phase. Conclusions: Upward biases in POC compared with venous plasma HPLC-MS/MS were likely due to higher paracetamol concentrations in capillary blood than in venous plasma and to faulty individual sensors. The novel POC method is a promising tool for paracetamol concentration analysis.
Description
The authors would like to thank A/Prof. Mark Chatfield, University of Queensland, Australia, for his assistance with Stata code for Bland–Altman and Passing-Bablok analysis; and Laura Tervala, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland; and Meeri Alanappa, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland, for their skilful assistance.
Keywords
acetaminophen, capillary, diagnostics, intoxication, paracetamol, point-of-care, SWCNT
Other note
Citation
Kujala , J , Wester , N , Lohela , T J , Kurkela , M , Backman , J T , Mikladal , B , Laurila , T , Koskinen , J , Lilius , T O & Kalso , E A 2023 , ' Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples ' , British Journal of Clinical Pharmacology , vol. 89 , no. 9 , pp. 2933-2938 . https://doi.org/10.1111/bcp.15794