Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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en
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6
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British Journal of Clinical Pharmacology, Volume 89, issue 9, pp. 2933-2938
Abstract
Aims: Measuring venous plasma paracetamol concentrations is time- and resource-consuming. We aimed to validate a novel electrochemical point-of-care (POC) assay for rapid paracetamol concentration determinations. Methods: Twelve healthy volunteers received 1 g oral paracetamol, and its concentrations were analysed 10 times over 12 h for capillary whole blood (POC), venous plasma (high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS)), and dried capillary blood (HPLC-MS/MS). Results: At concentrations >30 μM, POC showed upward biases of 20% (95% limits of agreement [LOA] −22 to 62) and 7% (95% LOA −23 to 38) compared with venous plasma and capillary blood HPLC-MS/MS, respectively. There were no significant differences between mean concentrations for the paracetamol elimination phase. Conclusions: Upward biases in POC compared with venous plasma HPLC-MS/MS were likely due to higher paracetamol concentrations in capillary blood than in venous plasma and to faulty individual sensors. The novel POC method is a promising tool for paracetamol concentration analysis.Description
The authors would like to thank A/Prof. Mark Chatfield, University of Queensland, Australia, for his assistance with Stata code for Bland–Altman and Passing-Bablok analysis; and Laura Tervala, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland; and Meeri Alanappa, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland, for their skilful assistance.
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Kujala, J, Wester, N, Lohela, T J, Kurkela, M, Backman, J T, Mikladal, B, Laurila, T, Koskinen, J, Lilius, T O & Kalso, E A 2023, 'Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples', British Journal of Clinical Pharmacology, vol. 89, no. 9, pp. 2933-2938. https://doi.org/10.1111/bcp.15794