Core–Shell Structured Fluorescent Protein Nanoparticles: New Paradigm Toward Zero-Thermal-Quenching in High-Power Biohybrid Light-Emitting Diodes

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2023-06-02
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Mcode
Degree programme
Language
en
Pages
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Advanced Science, Volume 10, issue 16
Abstract
Stable and efficient high-power biohybrid light-emitting diodes (Bio-HLEDs) using fluorescent proteins (FPs) in photon downconverting filters have not been achieved yet, reaching best efficiencies of 130 lm W−1 stable for >5 h. This is related to the rise of the device temperature (70–80 °C) caused by FP-motion and quick heat-transmission in water-based filters, they lead to a strong thermal emission quenching followed by the quick chromophore deactivation via photoinduced H-transfer. To tackle both issues at once, this work shows an elegant concept of a new FP-based nanoparticle, in which the FP core is shielded by a SiO2-shell (FP@SiO2) with no loss of the photoluminescence figures-of-merit over years in foreign environments: dry powder at 25 °C (ambient) or constant 50 °C, as well as suspensions in organic solvents. This enables the preparation of water-free photon downconverting coatings with FP@SiO2, realizing on-chip high-power Bio-HLEDs with 100 lm W−1 stable for >120 h. Both thermal emission quenchingand H-transfer deactivation are suppressed, since the device temperature holds <40 °C and remote high-power Bio-HLEDs exhibit final stabilities of 130 days compared to reference devices with water-based FP@SiO2 (83 days) and FP-polymer coatings (>100 h). Hence, FP@SiO2 is a new paradigm toward water-free zero-thermal-quenching biophosphors for first-class high-power Bio-HLEDs.
Description
Funding Information: R.D.C., M.P., S.F., and N.A.D. acknowledge the European Union's Horizon 2020 research and innovation ERC‐Co InOutBioLight No. 816856. M.N. and R.D.C. acknowledge FPNP‐BioLED No. 101022975 funded by H2020‐MSCA‐IF‐2020 of the European Commission and Dr. Van Opdenbosch for the X‐ray characterization assistance. A.E. and M.A.K. acknowledge the provision of facilities and technical support by Aalto University Bioeconomy Facilities and OtaNano‐Nanomicroscopy Center (Aalto‐NMC) J.R.B. acknowledges the Spanish MCIN/AIE/10.13039/501100011033, the “ERDF A way of making Europe”, the “European Union” (project PID2019‐109742GB‐I00). Publisher Copyright: © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
Keywords
biohybrid light-emitting diodes, fluorescent protein, hybrid protein-metal oxide nanoparticles, photon downconverting filters, protein-based lighting
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Citation
Nieddu , M , Patrian , M , Ferrara , S , Fuenzalida Werner , J P , Kohler , F , Anaya-Plaza , E , Kostiainen , M A , Dietz , H , Berenguer , J R & Costa , R D 2023 , ' Core–Shell Structured Fluorescent Protein Nanoparticles: New Paradigm Toward Zero-Thermal-Quenching in High-Power Biohybrid Light-Emitting Diodes ' , Advanced Science , vol. 10 , no. 16 , 2300069 . https://doi.org/10.1002/advs.202300069