Isolation of structurally distinct lignin–carbohydrate fractions from maize stem by sequential alkaline extractions and endoglucanase treatment
School of Chemical Technology | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Bioresource Technology, Volume 133
AbstractSequential fractionation of extractive-free maize stems was carried out using two mild alkaline extractions (0.5 and 2 M NaOH, 20 'C, 24 h) before and after endoglucanase treatment. This procedure provided two lignin–carbohydrate fractions (LC1 and LC2) recovered after each alkali treatment. LC1 and LC2 contained 39% and 8% of the total lignin amount, respectively. These two fractions contained structurally distinct lignin molecules. While the content of resistant interunit bonds in lignin was 77% in LC1, it was increased up to 98% in LC2. Not unexpectedly, both alkali-soluble fractions contained substantial amount of p-coumaric and ferulic acids ether-linked to lignins. These results outline heterogeneity of maize stem lignins related to fractionation of grass materials.
Lignin, isolation, extraction, alkaline, structure, thioacidolysis, GC/MS
Sipponen, Mika Henrikki & Lapierre, Catherine & Méchin, Valérie & Baumberger, Stéphanie. 2013. Isolation of structurally distinct lignin–carbohydrate fractions from maize stem by sequential alkaline extractions and endoglucanase treatment. Bioresource Technology. Volume 133. P. 522-528. ISSN 0960-8524 (printed). DOI: 10.1016/j.biortech.2013.01.175.