Isolation of structurally distinct lignin–carbohydrate fractions from maize stem by sequential alkaline extractions and endoglucanase treatment
Loading...
Journal Title
Journal ISSN
Volume Title
School of Chemical Technology |
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Unless otherwise stated, all rights belong to the author. You may download, display and print this publication for Your own personal use. Commercial use is prohibited.
Date
2013
Major/Subject
Mcode
Degree programme
Language
en
Pages
522-528
Series
Bioresource Technology, Volume 133
Abstract
Sequential fractionation of extractive-free maize stems was carried out using two mild alkaline extractions (0.5 and 2 M NaOH, 20 'C, 24 h) before and after endoglucanase treatment. This procedure provided two lignin–carbohydrate fractions (LC1 and LC2) recovered after each alkali treatment. LC1 and LC2 contained 39% and 8% of the total lignin amount, respectively. These two fractions contained structurally distinct lignin molecules. While the content of resistant interunit bonds in lignin was 77% in LC1, it was increased up to 98% in LC2. Not unexpectedly, both alkali-soluble fractions contained substantial amount of p-coumaric and ferulic acids ether-linked to lignins. These results outline heterogeneity of maize stem lignins related to fractionation of grass materials.Description
Keywords
Lignin, isolation, extraction, alkaline, structure, thioacidolysis, GC/MS
Citation
Sipponen, Mika Henrikki & Lapierre, Catherine & Méchin, Valérie & Baumberger, Stéphanie. 2013. Isolation of structurally distinct lignin–carbohydrate fractions from maize stem by sequential alkaline extractions and endoglucanase treatment. Bioresource Technology. Volume 133. P. 522-528. ISSN 0960-8524 (printed). DOI: 10.1016/j.biortech.2013.01.175.
