Fully inkjet-printed glucose assay fabricated on highly porous pigment coating

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dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorJutila, Eveliinaen_US
dc.contributor.authorKoivunen, Ristoen_US
dc.contributor.authorBollström, Rogeren_US
dc.contributor.authorGane, Patricken_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.groupauthorPrinting Technologyen
dc.contributor.organizationOmya AGen_US
dc.date.accessioned2020-06-01T06:54:05Z
dc.date.available2020-06-01T06:54:05Z
dc.date.issued2020-05-06en_US
dc.description.abstractA simple paper-based microfluidic device, fabricated on a highly porous coating consisting of functionalised calcium carbonate (FCC) pigment and a microfibrillated cellulose (MFC) and/or polyvinyl alcohol (PVOH) binder, was developed for glucose detection. Both the hydrophobic patterns, consisting of alkyl ketene dimer (AKD), and the enzyme ink, consisting of glucose oxidase (GOx), horseradish peroxidase (HRP) and an indicator containing 4-aminoantipyrine (4-AAP), and sodium 3,5-dichloro-2-hydroxy-benzenesulphonic acid (DHBS) were inkjet-printed. Multiple coating formulations were tested using two indicators, potassium iodide (KI) and 4-AAP/DHBS, to find the optimal formulation with regard to detection sensitivity and assay stability. Higher binder concentrations increased the enzyme activity, especially in the case of PVOH. Two coatings, containing either solely MFC or both MFC and PVOH were ultimately chosen for the glucose assay experiments. For the assays, a 42 nl volume of buffered enzyme solution, containing a total of 5.04 mU GOx and approximately 1.01 mU HRP, in combination with 4 mM 4-AAP and 8 mM DHBS, was printed, and a 1 µl glucose solution, made up over a range of concentrations, was applied onto the samples to evaluate the response. A good linearity was achieved between detection and glucose concentration between 0.1 and 0.6 mM with both coatings. The assays remained stable for four weeks when stored at − 20 °C. The results show that the functional coated substrates offer a viable alternative to cellulose-based substrates for microfluidic applications enabling the use of small reagent and sample volumes.en
dc.description.versionPeer revieweden
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationJutila, E, Koivunen, R, Bollström, R & Gane, P 2020, ' Fully inkjet-printed glucose assay fabricated on highly porous pigment coating ', Microfluidics and Nanofluidics, vol. 24, no. 6, 40 . https://doi.org/10.1007/s10404-020-02344-4en
dc.identifier.doi10.1007/s10404-020-02344-4en_US
dc.identifier.issn1613-4982
dc.identifier.otherPURE UUID: 9c3fdae0-9835-4164-a4e4-d844b6512ec0en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/9c3fdae0-9835-4164-a4e4-d844b6512ec0en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85084211505&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/43016178/CHEM_Jutila_et_al_2020_Fully_Inkjet_printedGlucose_MicroNano.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/44524
dc.identifier.urnURN:NBN:fi:aalto-202006013497
dc.language.isoenen
dc.publisherSpringer Verlag
dc.relation.ispartofseriesMicrofluidics and Nanofluidicsen
dc.relation.ispartofseriesVolume 24, issue 6en
dc.rightsopenAccessen
dc.subject.keywordGlucose assayen_US
dc.subject.keywordHighly porous coatingen_US
dc.subject.keywordMicrofluidic deviceen_US
dc.subject.keywordPaper microfluidicsen_US
dc.titleFully inkjet-printed glucose assay fabricated on highly porous pigment coatingen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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[article-cris] Kemian tekniikan korkeakoulu / CHEM