Catch the dream Wave, Propagation of Cortical Slow Oscillation to the Striatum in anaesthetised mice

Thumbnail Image
Files
master_Ferreira_Tiago_2014.pdf (2.42 MB)
Journal Title
Journal ISSN
Volume Title
Perustieteiden korkeakoulu | Master's thesis
Unless otherwise stated, all rights belong to the author. You may download, display and print this publication for Your own personal use. Commercial use is prohibited.
Author
Date
2014-11-03
Department
Major/Subject
Computational Systems Biology
Mcode
IL3013
Degree programme
Master's Degree Programme in Computational and Systems Biology (euSYSBIO)
Language
en
Pages
52
Series
Abstract
Under anaesthesia or in deep sleep, different parts of the brain have a distinctive slow oscillatory activity, characterised by states of high membrane potential and intensive spiking activity, the Up-states; followed by hyper-polarisation and quiescence, the Down-states. This activity has been previously described in vitro and in vivo in the cortex and the striatum, across several species. Here, we look into it, during anaesthesia, in the mouse brain. Using whole-cell patch-clamp recordings of cortical cells, it was possible to compare different signal processing methods used to extract the Up-and-Down states in extracellular recordings of the cortex. Our results show that the method based on the Multi-Unit Activity (> 200Hz) have better accuracy than High-Gamma Range (20 - 100Hz) or wavelet decomposition (< 2Hz band). After establishing the most robust method, this was used to compare the intracellular recordings of striatal cells to different parts of the cortex. The results obtained here support a functional connection between the dorsolateral striatal neurons and the ipsilateral barrel field. They also support a functional connection between dorsomedial striatal cells and the primary visual cortex. The analysis of delay between recordings allowed to establish temporal relationships between the contralateral barrel field, the ipsilateral barrel field, and the dorsolateral striatum; and between the ipsilateral barrel field, the ipsilateral primary visual field and the dorsomedial striatum.
Description
Supervisor
Rousu, Juho
Aurell, Erik
Thesis advisor
Reig, Ramon
Keywords
cortex, striatum, anaesthesia, slow oscillation, spontaneous activity, ketamine, in vivo, up states, wavelet, MUA, high-gamma range, MSNs
Other note
Citation
Permanent link to this item
https://urn.fi/URN:NBN:fi:aalto-201411123010
Collections
[dipl] Perustieteiden korkeakoulu / SCI