Silica–gentamicin nanohybrids: Combating antibiotic resistance, bacterial biofilms, and in vivo toxicity
Loading...
Access rights
openAccess
Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
This publication is imported from Aalto University research portal.
View publication in the Research portal
View/Open full text file from the Research portal
View publication in the Research portal
View/Open full text file from the Research portal
Date
2018
Major/Subject
Mcode
Degree programme
Language
en
Pages
19
7939-7957
7939-7957
Series
International journal of nanomedicine
Abstract
Introduction: Antibiotic resistance is a growing concern in health care. Methicillin-resistant Staphylococcus aureus (MRSA), forming biofilms, is a common cause of resistant orthopedic implant infections. Gentamicin is a crucial antibiotic preventing orthopedic infections. Silica–gentamicin (SiO -G) delivery systems have attracted significant interest in preventing the formation of biofilms. However, compelling scientific evidence addressing their efficacy against planktonic MRSA and MRSA biofilms is still lacking, and their safety has not extensively been studied. Materials and methods: In this work, we have investigated the effects of SiO2-G nanohybrids against planktonic MRSA as well as MRSA and Escherichia coli biofilms and then evaluated their toxicity in zebrafish embryos, which are an excellent model for assessing the toxicity of nanotherapeutics. Results: SiO -G nanohybrids inhibited the growth and killed planktonic MRSA at a minimum concentration of 500 g/mL. SiO2-G nanohybrids entirely eradicated E. coli cells in biofilms at a minimum concentration of 250 g/mL and utterly deformed their ultrastructure through the deterioration of bacterial shapes and wrinkling of their cell walls. Zebrafish embryos exposed to SiO2-G nanohybrids (500 and 1,000 g/mL) showed a nonsignificant increase in mortality rates, 13.4 9.4 and 15% 7.1%, respectively, mainly detected 24 hours post fertilization (hpf). Frequencies of malformations were significantly different from the control group only 24 hpf at the higher exposure concentration. Conclusion: Collectively, this work provides the first comprehensive in vivo assessment of SiO2-G nanohybrids as a biocompatible drug delivery system and describes the efficacy of SiO2-G nanohybrids in combating planktonic MRSA cells and eradicating E. coli biofilms.Description
Keywords
Other note
Citation
Ahmed Mosselhy Abdelrehiem, D, He, W, Hynönen, U, Meng, Y, Mohammadi, P, Palva, A, Feng, Q, Hannula, S-P, Nordström, K & Linder, M 2018, ' Silica–gentamicin nanohybrids : Combating antibiotic resistance, bacterial biofilms, and in vivo toxicity ', International journal of nanomedicine, pp. 7939-7957 . https://doi.org/10.2147/IJN.S182611
