Electrochemical Detection of Morphine in Untreated Human Capillary Whole Blood

Simple item page
dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorVerrinder, Elsien_US
dc.contributor.authorWester, Niklasen_US
dc.contributor.authorLeppänen, Ellien_US
dc.contributor.authorLilius, Tuomasen_US
dc.contributor.authorKalso, Eijaen_US
dc.contributor.authorMikladal, Bjørnen_US
dc.contributor.authorVarjos, Ilkkaen_US
dc.contributor.authorKoskinen, Jarien_US
dc.contributor.authorLaurila, Tomien_US
dc.contributor.departmentDepartment of Electrical Engineering and Automationen
dc.contributor.departmentDepartment of Chemistry and Materials Scienceen
dc.contributor.groupauthorMicrosystems Technologyen
dc.contributor.groupauthorPhysical Characteristics of Surfaces and Interfacesen
dc.contributor.organizationUniversity of Helsinkien_US
dc.contributor.organizationCanatu Oyen_US
dc.date.accessioned2021-06-16T06:55:31Z
dc.date.available2021-06-16T06:55:31Z
dc.date.issued2021-05-04en_US
dc.descriptionFunding Information: This work was supported by Business Finland (FEDOC 211637 and FEPOD 2117731 projects) and Aalto ELEC Doctoral School. Publisher Copyright: ©
dc.description.abstractDisposable single-use electrochemical sensor strips were used for quantitative detection of small concentrations of morphine in untreated capillary whole blood. Single-walled carbon nanotube (SWCNT) networks were fabricated on a polymer substrate to produce flexible, reproducible sensor strips with integrated reference and counter electrodes, compatible with industrial-scale processes. A thin Nafion coating was used on top of the sensors to enable direct electrochemical detection in whole blood. These sensors were shown to detect clinically relevant concentrations of morphine both in buffer and in whole blood samples. Small 38 μL finger-prick blood samples were spiked with 2 μL of morphine solution of several concentrations and measured without precipitation of proteins or any other further pretreatment. A linear range of 0.5-10 μM was achieved in both matrices and a detection limit of 0.48 μM in buffer. In addition, to demonstrate the applicability of the sensor in a point-of-care device, single-determination measurements were done with capillary samples from three subjects. An average recovery of 60% was found, suggesting that the sensor only measures the free, unbound fraction of the drug. An interference study with other opioids and possible interferents showed the selectivity of the sensor. This study clearly indicates that these Nafion/SWCNT sensor strips show great promise as a point-of-care rapid test for morphine in blood.en
dc.description.versionPeer revieweden
dc.format.extent7
dc.format.extent11563-11569
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationVerrinder, E, Wester, N, Leppänen, E, Lilius, T, Kalso, E, Mikladal, B, Varjos, I, Koskinen, J & Laurila, T 2021, ' Electrochemical Detection of Morphine in Untreated Human Capillary Whole Blood ', ACS Omega, vol. 6, no. 17, pp. 11563-11569 . https://doi.org/10.1021/acsomega.1c00773en
dc.identifier.doi10.1021/acsomega.1c00773en_US
dc.identifier.issn2470-1343
dc.identifier.otherPURE UUID: 3d186ef9-896a-4714-b4a7-7a51d5f8bf23en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/3d186ef9-896a-4714-b4a7-7a51d5f8bf23en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85106726283&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/64677475/ELEC_Verrinder_etal_Electrochemical_Detection_of_Morphine_ACS_Omega_2021_finalpublishedversion.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/108122
dc.identifier.urnURN:NBN:fi:aalto-202106167380
dc.language.isoenen
dc.publisherAMERICAN CHEMICAL SOCIETY
dc.relation.ispartofseriesACS Omegaen
dc.relation.ispartofseriesVolume 6, issue 17en
dc.rightsopenAccessen
dc.titleElectrochemical Detection of Morphine in Untreated Human Capillary Whole Blooden
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
Files
Collections
[article-cris] Kemian tekniikan korkeakoulu / CHEM