Electrochemical Detection of Morphine in Untreated Human Capillary Whole Blood
Loading...
Access rights
openAccess
publishedVersion
URL
Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
This publication is imported from Aalto University research portal.
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
Date
Major/Subject
Mcode
Degree programme
Language
en
Pages
7
Series
ACS Omega, Volume 6, issue 17, pp. 11563-11569
Abstract
Disposable single-use electrochemical sensor strips were used for quantitative detection of small concentrations of morphine in untreated capillary whole blood. Single-walled carbon nanotube (SWCNT) networks were fabricated on a polymer substrate to produce flexible, reproducible sensor strips with integrated reference and counter electrodes, compatible with industrial-scale processes. A thin Nafion coating was used on top of the sensors to enable direct electrochemical detection in whole blood. These sensors were shown to detect clinically relevant concentrations of morphine both in buffer and in whole blood samples. Small 38 μL finger-prick blood samples were spiked with 2 μL of morphine solution of several concentrations and measured without precipitation of proteins or any other further pretreatment. A linear range of 0.5-10 μM was achieved in both matrices and a detection limit of 0.48 μM in buffer. In addition, to demonstrate the applicability of the sensor in a point-of-care device, single-determination measurements were done with capillary samples from three subjects. An average recovery of 60% was found, suggesting that the sensor only measures the free, unbound fraction of the drug. An interference study with other opioids and possible interferents showed the selectivity of the sensor. This study clearly indicates that these Nafion/SWCNT sensor strips show great promise as a point-of-care rapid test for morphine in blood.Description
Funding Information: This work was supported by Business Finland (FEDOC 211637 and FEPOD 2117731 projects) and Aalto ELEC Doctoral School. Publisher Copyright: ©
Keywords
Other note
Citation
Verrinder, E, Wester, N, Leppänen, E, Lilius, T, Kalso, E, Mikladal, B, Varjos, I, Koskinen, J & Laurila, T 2021, 'Electrochemical Detection of Morphine in Untreated Human Capillary Whole Blood', ACS Omega, vol. 6, no. 17, pp. 11563-11569. https://doi.org/10.1021/acsomega.1c00773