Biomimetic collagen I and IV double layer Langmuir-Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorSorkio, Anni E.en_US
dc.contributor.authorVuorimaa-Laukkanen, Elina P.en_US
dc.contributor.authorHakola, Hanna M.en_US
dc.contributor.authorLiang, Huaminen_US
dc.contributor.authorUjula, Tiina A.en_US
dc.contributor.authorValle-Delgado, Juan Joseen_US
dc.contributor.authorOsterberg, Monikaen_US
dc.contributor.authorYliperttula, Marjo L.en_US
dc.contributor.authorSkottman, Helien_US
dc.contributor.departmentDepartment of Forest Products Technologyen
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.organizationUniversity of Tampereen_US
dc.contributor.organizationTampere University of Technologyen_US
dc.contributor.organizationAalto Universityen_US
dc.contributor.organizationUniversity of Helsinkien_US
dc.date.accessioned2017-05-11T09:09:42Z
dc.date.available2017-05-11T09:09:42Z
dc.date.embargoinfo:eu-repo/date/embargoEnd/2017-05-31en_US
dc.date.issued2015-05en_US
dc.description.abstractThe environmental cues received by the cells from synthetic substrates in vitro are very different from those they receive in vivo. In this study, we applied the LangmuireSchaefer (LS) deposition, a variant of LangmuireBlodgett technique, to fabricate a biomimetic microenvironment mimicking the structure and organization of native Bruch's membrane for the production of the functional human embryonic stem cell derived retinal pigment epithelial (hESC-RPE) cells. Surface pressure-area isotherms were measured simultaneously with Brewster angle microscopy to investigate the self-assembly of human collagens type I and IV on air-subphase interface. Furthermore, the structure of the prepared collagen LS films was characterized with scanning electron microscopy, atomic force microscopy, surface plasmon resonance measurements and immunofluorescent staining. The integrity of hESC-RPE on double layer LS films was investigated by measuring transepithelial resistance and permeability of small molecular weight substance. Maturation and functionality of hESC-RPE cells on double layer collagen LS films was further assessed by RPE-specific gene and protein expression, growth factor secretion, and phagocytic activity. Here, we demonstrated that the prepared collagen LS films have layered structure with oriented fibers corresponding to architecture of the uppermost layers of Bruch's membrane and result in increased barrier properties and functionality of hESC-RPE cells as compared to the commonly used dip-coated controls.en
dc.description.versionPeer revieweden
dc.format.extent13
dc.format.extent257-269
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationSorkio, A E, Vuorimaa-Laukkanen, E P, Hakola, H M, Liang, H, Ujula, T A, Valle-Delgado, J J, Osterberg, M, Yliperttula, M L & Skottman, H 2015, ' Biomimetic collagen I and IV double layer Langmuir-Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells ', Biomaterials, vol. 51, pp. 257-269 . https://doi.org/10.1016/j.biomaterials.2015.02.005en
dc.identifier.doi10.1016/j.biomaterials.2015.02.005en_US
dc.identifier.issn0142-9612
dc.identifier.issn1878-5905
dc.identifier.otherPURE UUID: dcfe8821-2e0b-416a-84f2-e86573da9e01en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/dcfe8821-2e0b-416a-84f2-e86573da9e01en_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/6736029/Accepted_paper.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/25905
dc.identifier.urnURN:NBN:fi:aalto-201705114280
dc.language.isoenen
dc.relation.ispartofseriesBIOMATERIALSen
dc.relation.ispartofseriesVolume 51en
dc.rightsopenAccessen
dc.subject.keywordBiomimetic materialen_US
dc.subject.keywordCollagen structureen_US
dc.subject.keywordLangmuir Blodgett filmen_US
dc.subject.keywordRetinal pigment epithelial cellen_US
dc.subject.keywordRetinaen_US
dc.subject.keywordHuman embryonic stem cellen_US
dc.titleBiomimetic collagen I and IV double layer Langmuir-Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cellsen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi

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