Graphene Biosensor Programming with Genetically Engineered Fusion Protein Monolayers

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2016-03-30
Major/Subject
Mcode
Degree programme
Language
en
Pages
8
8257-8264
Series
ACS Applied Materials and Interfaces, Volume 8, issue 12
Abstract
We demonstrate a label-free biosensor concept based on specific receptor modules, which provide immobilization and selectivity to the desired analyte molecules, and on charge sensing with a graphene field effect transistor. The receptor modules are fusion proteins in which small hydrophobin proteins act as the anchor to immobilize the receptor moiety. The functionalization of the graphene sensor is a single-step process based on directed self-assembly of the receptor modules on a hydrophobic surface. The modules are produced separately in fungi or plants and purified before use. The modules form a dense and well-oriented monolayer on the graphene transistor channel and the receptor module monolayer can be removed, and a new module monolayer with a different selectivity can be assembled in situ. The receptor module monolayers survive drying, showing that the functionalized devices can be stored and have a reasonable shelf life. The sensor is tested with small charged peptides and large immunoglobulin molecules. The measured sensitivities are in the femtomolar range, and the response is relatively fast, of the order of one second. (Graph Presented).
Description
Keywords
graphene, biosensor, fusion protein, hydrophobin, self-assembly, Debye length, CRYSTAL-STRUCTURES, HYDROPHOBIN HFBI, SENSORS, FUNCTIONALIZATION, PURIFICATION, FILMS
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Citation
Soikkeli, M, Kurppa, K, Kainlauri, M, Arpiainen, S, Paananen, A, Gunnarsson, D, Joensuu, J J, Laaksonen, P, Prunnila, M, Linder, M B & Ahopelto, J 2016, ' Graphene Biosensor Programming with Genetically Engineered Fusion Protein Monolayers ', ACS Applied Materials and Interfaces, vol. 8, no. 12, pp. 8257-8264 . https://doi.org/10.1021/acsami.6b00123