Mapping Nanocellulose- and Alginate-Based Photosynthetic Cell Factory Scaffolds : Interlinking Porosity, Wet Strength, and Gas Exchange
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2023-08-14
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en
Pages
14
3484-3497
3484-3497
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Biomacromolecules, Volume 24, issue 8
Abstract
To develop efficient solid-state photosynthetic cell factories for sustainable chemical production, we present an interdisciplinary experimental toolbox to investigate and interlink the structure, operative stability, and gas transfer properties of alginate- and nanocellulose-based hydrogel matrices with entrapped wild-type Synechocystis PCC 6803 cyanobacteria. We created a rheological map based on the mechanical performance of the hydrogel matrices. The results highlighted the importance of Ca2+-cross-linking and showed that nanocellulose matrices possess higher yield properties, and alginate matrices possess higher rest properties. We observed higher porosity for nanocellulose-based matrices in a water-swollen state via calorimetric thermoporosimetry and scanning electron microscopy imaging. Finally, by pioneering a gas flux analysis via membrane-inlet mass spectrometry for entrapped cells, we observed that the porosity and rigidity of the matrices are connected to their gas exchange rates over time. Overall, these findings link the dynamic properties of the life-sustaining matrix to the performance of the immobilized cells in tailored solid-state photosynthetic cell factories.Description
Funding Information: The work has been financially supported by the Academy of Finland (AlgaLEAF, project nos. 322752, 322754, and 322755). This project has been partially financed by the European Union’s Horizon 2020 research and innovation program under grant agreement no. 899576 (FuturoLEAF). T.L., V.R., M.M., T.T., T.C.M., and M.H. acknowledge the Academy of Finland’s Flagship Programme under project nos. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). Y.A. acknowledges the financial support by NordForsk NCoE program “NordAqua” (project 82845). J.P. and T.C.M. appreciate the financial support from the Foundation for Research of Natural Resources in Finland. | openaire: EC/H2020/899576/EU//FuturoLEAF
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Levä , T , Rissanen , V , Nikkanen , L , Siitonen , V , Heilala , M , Phiri , J , Maloney , T C , Kosourov , S , Allahverdiyeva , Y , Mäkelä , M & Tammelin , T 2023 , ' Mapping Nanocellulose- and Alginate-Based Photosynthetic Cell Factory Scaffolds : Interlinking Porosity, Wet Strength, and Gas Exchange ' , Biomacromolecules , vol. 24 , no. 8 , pp. 3484-3497 . https://doi.org/10.1021/acs.biomac.3c00261
