3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks
Loading...
Access rights
openAccess
Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
This publication is imported from Aalto University research portal.
View publication in the Research portal
View/Open full text file from the Research portal
View publication in the Research portal
View/Open full text file from the Research portal
Date
2021-12-01
Major/Subject
Mcode
Degree programme
Language
en
Pages
10
1098-1107
1098-1107
Series
International Journal of Biological Macromolecules, Volume 192
Abstract
Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.Description
The authors would like to acknowledge the Academy of Finland funding; No. 327248 (ValueBiomat) and 327865 (Bioeconomy). This work was a part of the Academy of Finland's Flagship Programme under Projects No. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). The authors would also like to thank Ms. Marja Kärkkäinen for providing TEMPO-CNF and the Biohybrid Materials Research Group (Aalto University) for providing the HepG2 cells.
Keywords
Other note
Citation
Bani Asadi, H, Teixeira Polez, R, Kimiaei, E, Madani, Z, Rojas Gaona, O, Österberg, M & Seppälä, J 2021, ' 3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks ', International Journal of Biological Macromolecules, vol. 192, pp. 1098-1107 . https://doi.org/10.1016/j.ijbiomac.2021.10.078
