Size-exclusion chromatography of cellulose : observations on the low-molar-mass fraction
Loading...
Access rights
openAccess
publishedVersion
URL
Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
This publication is imported from Aalto University research portal.
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
Other link related to publication (opens in new window)
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
Other link related to publication (opens in new window)
Authors
Date
2020-11
Department
Major/Subject
Mcode
Degree programme
Language
en
Pages
9
Series
Cellulose, Volume 27, issue 16, pp. 9217-9225
Abstract
Accurate determination of molar mass distribution for disperse cellulose samples has proved to be a challenging task. While size-exclusion chromatography coupled to multi-angle light scattering (MALS) and differential refractive index (DRI) detectors has become the most commonly used method for molar mass determination of celluloses, this technique suffers low sensitivity at the low-molar mass range. As discussed here, the universal method for accurate molar mass distribution analysis of cellulose samples not exists and thus thorough understanding on the differences of the various methodological approaches is important. In this study, the focus is in the accurate determination of the low-molar mass fraction. The results obtained by combining the two calibration strategies, MALS/DRI for polymeric region of a cellulose sample and conventional calibration for oligomeric region, was compared to the results obtained using only MALS/DRI (with extrapolation of the curve where signal-to-noise of MALS is low). For birch pulp sample, the results from the two approaches were comparable; it should be highlighted, however, that MALS/DRI slightly overestimates the molar masses at the low-molar-mass region.Description
Keywords
Cello-oligomers, Cellulose, Light-scattering detection, Oligomeric region, Size-exclusion chromatography
Other note
Citation
Pitkänen, L & Sixta, H 2020, ' Size-exclusion chromatography of cellulose : observations on the low-molar-mass fraction ', Cellulose, vol. 27, no. 16, pp. 9217-9225 . https://doi.org/10.1007/s10570-020-03419-9