Effect of Vitamin B2, B5, and B6 on the Growth of Methanococcus maripaludis

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Kemian tekniikan korkeakoulu | Bachelor's thesis
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Date

2025-08-30

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Mcode

CHEM3054

Degree programme

Aalto Bachelor's Programme in Science and Technology

Language

en

Pages

20+8

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Abstract

M. maripaludis is an obligate anaerobe and is often researched due to its unique methanogenic ability. The thesis aims to determine the significance of environmental vitamins B2, B5, and B6 on the growth of M. maripaludis. Methanococcus maripaludis JJ (DSMZ 2067) was grown using minimal McC medium and H2-CO2 substrates. The cultures were grown in a shaker at 37°C with 220 rpm. They were all supplied with the headspace gas CO2, H2, and H2S for approximately ten seconds and pressurized for about five more seconds. The cells were stored for one day to a week before inoculation. The results indicate that the vitamins, specifically B2 and B5, may not have pronounced effects on the growth of M. maripaludis if the cultures used are not under stress. It can be concluded that while old cells benefit from the addition of vitamins B2, B5, and B6, fresh cells, or cells not under any stress, may not display such behavior.

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Supervisor

Hummel, Michael

Thesis advisor

Telimaa, Heta

Keywords

environment, biofuel, methanococcus maripaludis, growth, vitamin, experiment

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https://urn.fi/URN:NBN:fi:aalto-202510077440

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[kand] Kemian tekniikan korkeakoulu / CHEM