Stability of DNA Origami Nanostructures in Physiological Media: The Role of Molecular Interactions
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A2 Katsausartikkeli tieteellisessä aikakauslehdessä
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2023-08-23
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Language
en
Pages
15
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Small, Volume 19, issue 34
Abstract
Programmable, custom-shaped, and nanometer-precise DNA origami nanostructures have rapidly emerged as prospective and versatile tools in bionanotechnology and biomedicine. Despite tremendous progress in their utilization in these fields, essential questions related to their structural stability under physiological conditions remain unanswered. Here, DNA origami stability is explored by strictly focusing on distinct molecular-level interactions. In this regard, the fundamental stabilizing and destabilizing ionic interactions as well as interactions involving various enzymes and other proteins are discussed, and their role in maintaining, modulating, or decreasing the structural integrity and colloidal stability of DNA origami nanostructures is summarized. Additionally, specific issues demanding further investigation are identified. This review – through its specific viewpoint – may serve as a primer for designing new, stable DNA objects and for adapting their use in applications dealing with physiological media.Description
Funding Information: V.L. and A.K. contributed equally to this work. The financial support through the Emil Aaltonen Foundation, the Sigrid Jusélius Foundation, the Jane and Aatos Erkko Foundation, the Finnish Academy of Science and Letters (Vilho, Yrjö, and Kalle Väisälä Fund), and ERA Chair MATTER from the European Union's Horizon 2020 research and innovation programme under Grant Agreement No. 856705 is gratefully acknowledged. Publisher Copyright: © 2023 The Authors. Small published by Wiley-VCH GmbH.
Keywords
DNA nanotechnology, enzymes, ions, nucleases, stability
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Citation
Linko, V & Keller, A 2023, ' Stability of DNA Origami Nanostructures in Physiological Media: The Role of Molecular Interactions ', Small, vol. 19, no. 34, 2301935 . https://doi.org/10.1002/smll.202301935