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Quantifying Subresolution 3D Morphology of Bone with Clinical Computed Tomography

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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en

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11

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Annals of Biomedical Engineering, Volume 48, issue 2, pp. 595-605

Abstract

The aim of this study was to quantify sub-resolution trabecular bone morphometrics, which are also related to osteoarthritis (OA), from clinical resolution cone beam computed tomography (CBCT). Samples (n = 53) were harvested from human tibiae (N = 4) and femora (N = 7). Grey-level co-occurrence matrix (GLCM) texture and histogram-based parameters were calculated from CBCT imaged trabecular bone data, and compared with the morphometric parameters quantified from micro-computed tomography. As a reference for OA severity, histological sections were subjected to OARSI histopathological grading. GLCM and histogram parameters were correlated to bone morphometrics and OARSI individually. Furthermore, a statistical model of combined GLCM/histogram parameters was generated to estimate the bone morphometrics. Several individual histogram and GLCM parameters had strong associations with various bone morphometrics (|r| > 0.7). The most prominent correlation was observed between the histogram mean and bone volume fraction (r = 0.907). The statistical model combining GLCM and histogram-parameters resulted in even better association with bone volume fraction determined from CBCT data (adjusted R-2 change = 0.047). Histopathology showed mainly moderate associations with bone morphometrics (|r| > 0.4). In conclusion, we demonstrated that GLCM- and histogram-based parameters from CBCT imaged trabecular bone (ex vivo) are associated with sub-resolution morphometrics. Our results suggest that sub-resolution morphometrics can be estimated from clinical CBCT images, associations becoming even stronger when combining histogram and GLCM-based parameters.

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| openaire: EC/H2020/336267/EU//3D-OA-HISTO

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Karhula, S S, Finnila, M A J, Rytky, S J O, Cooper, D M, Thevenot, J, Valkealahti, M, Pritzker, K P H, Haapea, M, Joukainen, A, Lehenkari, P, Kröger, H, Korhonen, R K, Nieminen, H J & Saarakkala, S 2020, 'Quantifying Subresolution 3D Morphology of Bone with Clinical Computed Tomography', Annals of Biomedical Engineering, vol. 48, no. 2, pp. 595-605. https://doi.org/10.1007/s10439-019-02374-2

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