Signal Amplification in Electrochemical DNA Biosensors Using Target-Capturing DNA Origami Tiles

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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ACS Sensors, Volume 8, issue 4
Electrochemical DNA (e-DNA) biosensors are feasible tools for disease monitoring, with their ability to translate hybridization events between a desired nucleic acid target and a functionalized transducer, into recordable electrical signals. Such an approach provides a powerful method of sample analysis, with a strong potential to generate a rapid time to result in response to low analyte concentrations. Here, we report a strategy for the amplification of electrochemical signals associated with DNA hybridization, by harnessing the programmability of the DNA origami method to construct a sandwich assay to boost charge transfer resistance (RCT) associated with target detection. This allowed for an improvement in the sensor limit of detection by two orders of magnitude compared to a conventional label-free e-DNA biosensor design and linearity for target concentrations between 10 pM and 1 nM without the requirement for probe labeling or enzymatic support. Additionally, this sensor design proved capable of achieving a high degree of strand selectivity in a challenging DNA-rich environment. This approach serves as a practical method for addressing strict sensitivity requirements necessary for a low-cost point-of-care device.
Funding Information: Financial support from EPSRC DTP (grant EP/R513349/1), the Emil Aaltonen Foundation, the Sigrid Jusélius Foundation, the Jane and Aatos Erkko Foundation, the Finnish Cultural Foundation (Kalle and Dagmar Välimaa Fund), the Magnus Ehrnrooth Foundation, and ERA Chair MATTER from the European Union’s Horizon 2020 research and innovation program under grant agreement no. 856705 is gratefully acknowledged. This work was carried out under the Academy of Finland Centers of Excellence Program (2022–2029) in Life-Inspired Hybrid Materials (LIBER), project number 346110. We acknowledge the provision of facilities and technical support by Aalto University Bioeconomy Facilities, OtaNano Nanomicroscopy Center (Aalto-NMC), and Micronova Nanofabrication Center. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.
antimicrobial resistance gene, DNA hybridization, DNA nanotechnology, electrochemical impedance spectroscopy, point-of-care devices, sensitivity enhancement, target selectivity
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Williamson, P, Piskunen, P, Ijäs, H, Butterworth, A, Linko, V & Corrigan, D K 2023, ' Signal Amplification in Electrochemical DNA Biosensors Using Target-Capturing DNA Origami Tiles ', ACS Sensors, vol. 8, no. 4, pp. 1471–1480 .