Biopolymer-Capped Pyrazinamide-Loaded Colloidosomes : In Vitro Characterization and Bioavailability Studies

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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en

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ACS Omega, Volume 8, issue 28, pp. 25515–25524

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This study aimed to prepare colloidosome particles loaded with pyrazinamide (PZA). These drug-loaded colloidosomes were prepared using an in situ gelation technique using a central composite design with a shell made of calcium carbonate (CaCO3) particles. Optimal amounts of 150 mg of CaCO3, sodium alginate (2%), and 400 mg of poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) concentration resulted in the maximum drug loading and efficient release profile. Field emission scanning electron microscopy results showed spherical porous particles with a good coating of the PHBV polymer. Additionally, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric and differential thermal analysis (TGA-DTA), and X-ray diffraction (XRD) analysis showed good compatibility between the drug and excipients. The pharmacokinetic studies demonstrated that the drug-loaded colloidosomes resulted in 4.26 times higher plasma drug concentrations with Cmax values of 32.386 ± 2.744 mcg/mL (PZA solution) and 115.868 ± 53.581 mcg/mL (PZA-loaded colloidosomes) and AUC0-t values of 61.24 mcg-h/mL (PZA solution) and 260.9 mcg-h/mL (PZA-loaded colloidosomes), indicating that colloidosomes have the potential to be effective drug carriers for delivering PZA to the target site.

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Funding Information: A.S. acknowledges the financial assistance provided by the Department of Science and Technology, Government of India, under Inspire Programme (IF: 180432). The authors acknowledge the central instrument facility of the Birla Institute of Technology. The graphical abstract has been created using Biorender. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.

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Singh, A, Das, S S, Ruokolainen, J, Kesari, K K & Singh, S K 2023, 'Biopolymer-Capped Pyrazinamide-Loaded Colloidosomes : In Vitro Characterization and Bioavailability Studies', ACS Omega, vol. 8, no. 28, pp. 25515–25524. https://doi.org/10.1021/acsomega.3c03135