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A synthetically modified hydrophobin showing enhanced fluorous affinity

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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en

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8

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Journal of Colloid and Interface Science, Volume 448, pp. 140-147

Abstract

Hydrophobins are natural surfactant proteins endowed with exceptional surface activity and film-forming capabilities and their use as effective “fluorine-free fluorosurfactants” has been recently reported. In order to increase their fluorophilicity further, here we report the preparation of a unique fluorous-modified hydrophobin, named F-HFBI. F-HFBI was found to be more effective than its wild-type parent protein HFBI at reducing interface tension of water at both air/water and oil/water interfaces, being particularly effective at the fluorous/water interface. F-HFBI was also found to largely retain the exceptionally good capability of forming strong and elastic films, typical of the hydrophobin family. Further studies by interface shear rheology and isothermal compression, alongside Quartz Crystal Microbalance and Atomic Force Microscopy, demonstrated the tendency of F-HFBI to form thicker films compared to the wild-type protein. These results suggest that F-HFBI may function as an effective compatibilizer for biphasic systems comprising a fluorous phase.

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Milani, R, Pirrie, L, Gazzera, L, Paananen, A, Baldrighi, M, Monogioudi, E, Cavallo, G, Linder, M, Resnati, G & Metrangolo, P 2015, 'A synthetically modified hydrophobin showing enhanced fluorous affinity', Journal of Colloid and Interface Science, vol. 448, pp. 140-147. https://doi.org/10.1016/j.jcis.2015.02.003

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