Impact of loosenins on the enzymatic preparation of cellulose nanocrystals
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Carbohydrate Polymers, Volume 357
Abstract
Whereas the enzymatic deconstruction of lignocellulosic materials is well established, comparatively few studies investigate the application of enzymes in bio-based material manufacturing. In this study, we demonstrate the potential of an ancestral endoglucanase from Bacillus subtilis (LFCA_EG) together with a loosenin from the white-rot fungus Phanerochaete carnosa (PcaLOOL12) to produce cellulose nanocrystals (CNC) with smaller size and higher yield than CNCs prepared using LFCA_EG alone. Moreover, CNCs prepared using both LFCA_EG and PcaLOOL12 retained the chemical purity, crystallinity, and thermal stability of previously described enzymatically prepared CNCs, and could be used to exfoliate graphite to generate low resistance, graphene-based conductive inks. Accordingly, this study highlights the potential of loosenins such as PcaLOOL12 to not only enhance the enzymatic deconstruction of lignocellulose but also the preparation of value-added cellulosic materials.Description
| openaire: EC/H2020/964764/EU//BioUPGRADE
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Jabalera, Y, Dahiya, D, Cencerrado, C D O, Caballero, A J, Zaldua, N, Eceiza, A, Master, E R & Perez-Jimenez, R 2025, 'Impact of loosenins on the enzymatic preparation of cellulose nanocrystals', Carbohydrate Polymers, vol. 357, 123469. https://doi.org/10.1016/j.carbpol.2025.123469