Computer-Aided Design of A-Trail Routed Wireframe DNA Nanostructures with Square Lattice Edges
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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en
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10
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ACS Nano, Volume 17, issue 7, pp. 6565–6574
Abstract
In recent years, interest in wireframe DNA origami has increased, with different designs, software, and applications emerging at a fast pace. It is now possible to design a wide variety of shapes by starting with a 2D or 3D mesh and using different scaffold routing strategies. The design choices of the edges in wireframe structures can be important in some applications and have already been shown to influence the interactions between nanostructures and cells. In this work, we increase the alternatives for the design of A-trail routed wireframe DNA structures by using four-helix bundles (4HB). Our approach is based on the incorporation of additional helices to the edges of the wireframe structure to create a 4HB on a square lattice. We first developed the software for the design of these structures, followed by a demonstration of the successful design and folding of a library of structures, and then, finally, we investigated the higher mechanical rigidity of the reinforced structures. In addition, the routing of the scaffold allows us to easily incorporate these reinforced edges together with more flexible, single helix edges, thereby allowing the user to customize the desired stiffness of the structure. We demonstrated the successful folding of this type of hybrid structure and the different stiffnesses of the different parts of the nanostructures using a combination of computational and experimental techniques.Description
Funding Information: The TEM data were collected at the Karolinska Institutet 3D-EM facility. We acknowledge support from the European Commission H2020 MSCA ITN (DNA Robotics GA No. 765703) to M.L. and B.H., from the Göran Gustafson foundation for Research in Natural Sciences and Medicine to B.H., from the Knut and Alice Wallenberg foundation (Grants KAW 2017.0114 and KAW 2017.0276) to B.H., from the Academy of Finland (grant no. 341908) to B.S., and from the Swedish Research Council to B.H. (grant no. 2019-01474). Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.
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Lolaico, M, Blokhuizen, S, Shen, B, Wang, Y & Högberg, B 2023, 'Computer-Aided Design of A-Trail Routed Wireframe DNA Nanostructures with Square Lattice Edges', ACS Nano, vol. 17, no. 7, pp. 6565–6574. https://doi.org/10.1021/acsnano.2c11982