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Scaling Up DNA Origami Lattice Assembly
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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en
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8
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Chemistry: A European Journal, Volume 27, issue 33, pp. 8564-8571
Abstract
The surface-assisted hierarchical assembly of DNA origami nanostructures is a promising route to fabricate regular nanoscale lattices. In this work, the scalability of this approach is explored and the formation of a homogeneous polycrystalline DNA origami lattice at the mica-electrolyte interface over a total surface area of 18.75 cm2 is demonstrated. The topological analysis of more than 50 individual AFM images recorded at random locations over the sample surface showed only minuscule and random variations in the quality and order of the assembled lattice. The analysis of more than 450 fluorescence microscopy images of a quantum dot-decorated DNA origami lattice further revealed a very homogeneous surface coverage over cm2 areas with only minor boundary effects at the substrate edges. At total DNA costs of € 0.12 per cm2, this large-scale nanopatterning technique holds great promise for the fabrication of functional surfaces.
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Funding Information: This work was supported by the Emil Aaltonen Foundation, the Jane and Aatos Erkko Foundation, the Sigrid Jusélius Foundation, and the Vilho, Yrjö and Kalle Väisälä Foundation of the Finnish Academy of Science and Letters. We acknowledge the provision of facilities and technical support by Aalto University Bioeconomy Facilities. This work has been partially funded by the Agencia Estatal de Investigación (AEI, 10.13039/501100011033) under grant PID2019‐106339GB‐I00 (to M.C.). Open access funding enabled and organized by Projekt DEAL. Publisher Copyright: © 2021 The Authors. Published by Wiley-VCH GmbH Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
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Xin, Y, Shen, B, Kostiainen, M A, Grundmeier, G, Castro, M, Linko, V & Keller, A 2021, 'Scaling Up DNA Origami Lattice Assembly', Chemistry: A European Journal, vol. 27, no. 33, pp. 8564-8571. https://doi.org/10.1002/chem.202100784