Spidroins under the Influence of Alcohol: Effect of Ethanol on Secondary Structure and Molecular Level Solvation of Silk-Like Proteins

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorTolmachev, Dmitry
dc.contributor.authorMalkamäki, Maaria
dc.contributor.authorLinder, Markus
dc.contributor.authorSammalkorpi, Maria
dc.contributor.departmentDepartment of Chemistry and Materials Science
dc.contributor.departmentDepartment of Bioproducts and Biosystems
dc.contributor.departmentDepartment of Chemistry and Materials Scienceen
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.date.accessioned2024-01-04T08:53:45Z
dc.date.available2024-01-04T08:53:45Z
dc.date.issued2023-12-11
dc.description.abstractFuture sustainable materials based on designer biomolecules require control of the solution assembly, but also interfacial interactions. Alcohol treatments of protein materials are an accessible means to this, making understanding of the process at the molecular level of seminal importance. We focus here on the influence of ethanol on spidroins, the main proteins of silk. By large-scale atomistically detailed molecular dynamics (MD) simulations and interconnected experiments, we characterize the protein aggregation, secondary structure changes, molecular level origins of them, and solvation environment changes for the proteins, as induced by ethanol as a solvation additive. The MD and circular dichoroism (CD) findings jointly show that ethanol promotes ordered structure in the protein molecules, leading to an increase of helix content and turns but also increased aggregation, as revealed by dynamic light scattering (DLS) and light microscopy. The structural changes correlate at the molecular level with increased intramolecular hydrogen bonding. The simulations reveal that polar amino acids, such as glutamine and serine, are most influenced by ethanol, whereas glycine residues are most prone to be involved in the ethanol-induced secondary structure changes. Furthermore, ethanol engages in interactions with the hydrophobic alanine-rich regions of the spidroin, significantly decreasing the hydrophobic interactions of the protein with itself and its surroundings. The protein solutes also change the microstructure of water/ethanol mixtures, essentially decreasing the level of larger local clustering. Overall, the work presents a systematic characterization of ethanol effects on a widely used, common protein type, spidroins, and generalizes the findings to other intrinsically disordered proteins by pinpointing the general features of the response. The results can aid in designing effective alcohol treatments for proteins, but also enable design and tuning of protein material properties by a relatively controllable solvation handle, the addition of ethanol.en
dc.description.versionPeer revieweden
dc.format.extent5638–5653
dc.format.mimetypeapplication/pdf
dc.identifier.citationTolmachev , D , Malkamäki , M , Linder , M & Sammalkorpi , M 2023 , ' Spidroins under the Influence of Alcohol: Effect of Ethanol on Secondary Structure and Molecular Level Solvation of Silk-Like Proteins ' , Biomacromolecules , vol. 24 , no. 12 , pp. 5638–5653 . https://doi.org/10.1021/acs.biomac.3c00637en
dc.identifier.doi10.1021/acs.biomac.3c00637
dc.identifier.issn1525-7797
dc.identifier.issn1526-4602
dc.identifier.otherPURE UUID: 52b5e661-2782-486c-a850-059b4b6b0951
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/52b5e661-2782-486c-a850-059b4b6b0951
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85179601909&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/130987195/CHEM_Tolmachev_et_al_Spidroins_under_2023_Biomacromolecules.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/125418
dc.identifier.urnURN:NBN:fi:aalto-202401041107
dc.language.isoenen
dc.publisherAmerican Chemical Society
dc.relation.ispartofseriesBiomacromoleculesen
dc.relation.ispartofseriesVolume 24, issue 12en
dc.rightsopenAccessen
dc.titleSpidroins under the Influence of Alcohol: Effect of Ethanol on Secondary Structure and Molecular Level Solvation of Silk-Like Proteinsen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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