Modularity impacts cellulose surface oxidation by a lytic polysaccharide monooxygenase from Streptomyces coelicolor

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2023-11
Major/Subject
Mcode
Degree programme
Language
en
Pages
12
10783-10794
Series
Cellulose, Volume 30, issue 17
Abstract
Lytic polysaccharide monooxygenases (LPMOs) catalyze the oxidation of β-(1,4)-linked polysaccharides, such as cellulose, in a reaction that requires an electron donor and H2O2 as co-substrate. Several LPMOs include a carbohydrate-binding module (CBM), which promotes action on insoluble substrates. Herein, a fluorescent labeling technique was used to track LPMO action on microcrystalline cellulose and evaluate the impact of CBMs on the distribution of LPMO activity across the fiber surface. Confocal microscopic images revealed that the distribution of oxidized positions on the cellulose surface was CBM-dependent: fluorescent spots were concentrated in reactions with a CBM-containing LPMO whereas they were more dispersed for a CBM-deficient LPMO variant. The more dispersed oxidation pattern for the CBM-free LPMO coincided with the release of fewer soluble reaction products.
Description
Open access funding provided by Norwegian University of Life Sciences. Contributions from O.R. and E.R.M. were financially supported by the Genome Canada and Ontario Genomics project: Synbiomics (Project Number 10405), the Research Council of Norway through grant 262853 to V.G.H.E. and the Novo Nordisk Foundation through grant NNF18OC0055736 to Z.F. also supported this work. Funding Information: The authors would like to acknowledge the financial support from Genome Canada, the Ontario Genomics project, the Research Council of Norway, and the Novo Nordisk Foundation. Publisher Copyright: © 2023, The Author(s).
Keywords
Aldonic acid, Carbohydrate-binding module (CBM), Cellulose, Fluorescence, Lytic polysaccharide monooxygenase (LPMO), Surface oxidation
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Citation
Raji , O , Eijsink , V G H , Master , E & Forsberg , Z 2023 , ' Modularity impacts cellulose surface oxidation by a lytic polysaccharide monooxygenase from Streptomyces coelicolor ' , Cellulose , vol. 30 , no. 17 , pp. 10783-10794 . https://doi.org/10.1007/s10570-023-05551-8