In vivo calcium imaging of evoked calcium waves in the embryonic cortex

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorYuryev, Mikhailen_US
dc.contributor.authorPellegrino, Christopheen_US
dc.contributor.authorJokinen, Villeen_US
dc.contributor.authorAndriichuk, Liliiaen_US
dc.contributor.authorKhirug, Stanislaven_US
dc.contributor.authorKhiroug, Leonarden_US
dc.contributor.authorRivera, Claudioen_US
dc.contributor.departmentDepartment of Materials Science and Engineeringen
dc.contributor.departmentDepartment of Chemistry and Materials Scienceen
dc.contributor.organizationUniversity of Helsinkien_US
dc.contributor.organizationAix-Marseille Universitéen_US
dc.date.accessioned2017-05-11T09:10:14Z
dc.date.available2017-05-11T09:10:14Z
dc.date.issued2016-01-06en_US
dc.description.abstractThe dynamics of intracellular calcium fluxes are instrumental in the proliferation, differentiation, and migration of neuronal cells. Knowledge thus far of the relationship between these calcium changes and physiological processes in the developing brain has derived principally from ex vivo and in vitro experiments. Here, we present a new method to image intracellular calcium flux in the cerebral cortex of live rodent embryos, whilst attached to the dam through the umbilical cord. Using this approach we demonstrate induction of calcium waves by laser stimulation. These waves are sensitive to ATP-receptor blockade and are significantly increased by pharmacological facilitation of intracellular-calcium release. This approach is the closest to physiological conditions yet achieved for imaging of calcium in the embryonic brain and as such opens new avenues for the study of prenatal brain development. Furthermore, the developed method could open the possibilities of preclinical translational studies in embryos particularly important for developmentally related diseases such as schizophrenia and autism.en
dc.description.versionPeer revieweden
dc.format.extent9
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationYuryev, M, Pellegrino, C, Jokinen, V, Andriichuk, L, Khirug, S, Khiroug, L & Rivera, C 2016, ' In vivo calcium imaging of evoked calcium waves in the embryonic cortex ', FRONTIERS IN CELLULAR NEUROSCIENCE, vol. 9, no. JAN2016, 500 . https://doi.org/10.3389/fncel.2015.00500en
dc.identifier.doi10.3389/fncel.2015.00500en_US
dc.identifier.issn1662-5102
dc.identifier.otherPURE UUID: e15b5cba-8feb-4152-87a4-1e8c98f025c3en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/e15b5cba-8feb-4152-87a4-1e8c98f025c3en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=84957831085&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/11561840/Yuryev_et_al_2016_In_vivo_calcium_imaging.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/25918
dc.identifier.urnURN:NBN:fi:aalto-201705114293
dc.language.isoenen
dc.relation.ispartofseriesFRONTIERS IN CELLULAR NEUROSCIENCEen
dc.relation.ispartofseriesVolume 9, issue JAN2016en
dc.rightsopenAccessen
dc.subject.keywordin vivo imagingen_US
dc.subject.keywordcalcium imagingen_US
dc.subject.keywordcortical developmenten_US
dc.subject.keywordtwo-photon microscopyen_US
dc.subject.keywordpurinergic receptorsen_US
dc.subject.keywordMOUSE CEREBRAL-CORTEXen_US
dc.subject.keywordDEVELOPING NEOCORTEXen_US
dc.subject.keywordNEURONAL MIGRATIONen_US
dc.subject.keywordSYNCHRONIZED ACTIVITYen_US
dc.subject.keywordELECTRICAL-ACTIVITYen_US
dc.subject.keywordCELLSen_US
dc.subject.keywordPATTERNSen_US
dc.subject.keywordREGISTRATIONen_US
dc.subject.keywordMODULATEen_US
dc.subject.keywordDRIVENen_US
dc.titleIn vivo calcium imaging of evoked calcium waves in the embryonic cortexen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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