Hydrophobin-Coated Echogenic Microbubbles for Molecular Targeting of Tumor Cells

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date

2025-06-12

Department

Department of Applied Physics
OtaNano

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Language

en

Pages

Series

Advanced Science, Volume 12, issue 22

Abstract

Cancer is a leading cause of death globally. Early detection of tumors can be an effective way to reduce mortality, however, traditional cancer diagnostic methods are based on the detection of organ dysfunction, which generally occurs at advanced stages of the disease. The first signs of disease initiation appear much earlier as molecular abnormalities yet are challenging to detect. An advanced design of microbubbles is reported that allows molecular targeting for subsequent binding to tumor cells. The microbubbles consist of perfluorocarbon gas encapsulated in a functional and stable shell made of a surface-active protein (hydrophobin HFBI from Trichoderma reesei) and corresponding fusion protein (HFBI-domZ) onto which antibodies can be easily grafted. The functionality of the bubbles is investigated, among others, using cryogenic transmission electron microscopy (cryo-TEM), confocal microscopy, and in vitro experiments. The results show the potential of the microbubbles as a probe to be used as a targeting contrast agent in ultrasound molecular imaging (MI) for cancer diagnostics.

Description

Publisher Copyright: © 2025 The Author(s). Advanced Science published by Wiley-VCH GmbH.

Keywords

hydrophobins, molecular imaging, targeted microbubbles, tumor cells, ultrasound contrast agents

Other note

DOI

10.1002/advs.202401526

Citation

Al-Terke, H H, Beaune, G, Junaid, M, Seitsonen, J, Paananen, A, Metrangolo, P, Timonen, J V I, Joensuu, J & Ras, R H A 2025, 'Hydrophobin-Coated Echogenic Microbubbles for Molecular Targeting of Tumor Cells', Advanced Science, vol. 12, no. 22, 2401526. https://doi.org/10.1002/advs.202401526

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https://urn.fi/URN:NBN:fi:aalto-202507015398

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[article-cris] Perustieteiden korkeakoulu / SCI