Tropomyosin Tpm3.1 Is Required to Maintain the Structure and Function of the Axon Initial Segment

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date

2020-05-22

Department

Department of Computer Science

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Language

en

Pages

50

Series

iScience, Volume 23, issue 5

Abstract

The axon initial segment (AIS) is the site of action potential initiation and serves as a cargo transport filter and diffusion barrier that helps maintain neuronal polarity. The AIS actin cytoskeleton comprises actin patches and periodic sub-membranous actin rings. We demonstrate that tropomyosin isoform Tpm3.1 co-localizes with actin patches and that the inhibition of Tpm3.1 led to a reduction in the density of actin patches. Furthermore, Tpm3.1 showed a periodic distribution similar to sub-membranous actin rings but Tpm3.1 was only partially congruent with sub-membranous actin rings. Nevertheless, the inhibition of Tpm3.1 affected the uniformity of the periodicity of actin rings. Furthermore, Tpm3.1 inhibition led to reduced accumulation of AIS structural and functional proteins, disruption in sorting somatodendritic and axonal proteins, and a reduction in firing frequency. These results show that Tpm3.1 is necessary for the structural and functional maintenance of the AIS.

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Keywords

Biological Sciences, Cell Biology, Cellular Neuroscience, Molecular Neuroscience

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DOI

10.1016/j.isci.2020.101053

Citation

Abouelezz, A, Stefen, H, Segerstråle, M, Micinski, D, Minkeviciene, R, Lahti, L, Hardeman, E C, Gunning, P W, Hoogenraad, C C, Taira, T, Fath, T & Hotulainen, P 2020, 'Tropomyosin Tpm3.1 Is Required to Maintain the Structure and Function of the Axon Initial Segment', iScience, vol. 23, no. 5, 101053. https://doi.org/10.1016/j.isci.2020.101053

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https://urn.fi/URN:NBN:fi:aalto-202006013479

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[article-cris] Perustieteiden korkeakoulu / SCI