Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays

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openAccess

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Volume Title

A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Date

2020-12-17

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Mcode

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Language

en

Pages

9

Series

Small

Abstract

Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between the cellulosic nanogel and human immunoglobulin G as well as bovine serum albumin (BSA) are investigated. Confocal microscopy, electroacoustic microgravimetry, and surface plasmon resonance are used to access information about the adsorption behavior and viscoelastic properties of self-assembled nanogels. A significant BSA adsorption capacity on nanogel layers (17 mg m−2) is measured, 300% higher compared to typical polymer coatings. This high protein affinity further confirms the promise of the introduced colloidal gel layer, in increasing sensitivity and advancing a new generation of substrates for a variety of applications, including immunoassays, as demonstrated in this work.

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| openaire: EC/H2020/760876/EU//INNPAPER | openaire: EC/H2020/788489/EU//BioELCell

Keywords

amorphous nanocellulose, cellulose II nanogel, colloids, core/shell nanoparticles, immunosensors, protein adsorption

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Citation

Solin, K, Beaumont, M, Rosenfeldt, S, Orelma, H, Borghei, M, Bacher, M, Opietnik, M & Rojas, O J 2020, ' Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays ', Small, vol. 16, no. 50, 2004702 . https://doi.org/10.1002/smll.202004702