Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2020-12-17
Major/Subject
Mcode
Degree programme
Language
en
Pages
9
Series
Small
Abstract
Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between the cellulosic nanogel and human immunoglobulin G as well as bovine serum albumin (BSA) are investigated. Confocal microscopy, electroacoustic microgravimetry, and surface plasmon resonance are used to access information about the adsorption behavior and viscoelastic properties of self-assembled nanogels. A significant BSA adsorption capacity on nanogel layers (17 mg m−2) is measured, 300% higher compared to typical polymer coatings. This high protein affinity further confirms the promise of the introduced colloidal gel layer, in increasing sensitivity and advancing a new generation of substrates for a variety of applications, including immunoassays, as demonstrated in this work.
Description
| openaire: EC/H2020/760876/EU//INNPAPER | openaire: EC/H2020/788489/EU//BioELCell
Keywords
amorphous nanocellulose, cellulose II nanogel, colloids, core/shell nanoparticles, immunosensors, protein adsorption
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Citation
Solin , K , Beaumont , M , Rosenfeldt , S , Orelma , H , Borghei , M , Bacher , M , Opietnik , M & Rojas , O J 2020 , ' Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays ' , Small , vol. 16 , no. 50 , 2004702 . https://doi.org/10.1002/smll.202004702